التفاصيل البيبلوغرافية
| العنوان: |
Isolation and Molecular Detection of Mycoplasma ovipneumoniae and Mycoplasma arginini in Sheep and Goats in Karnataka, India. |
| المؤلفون: |
Karthik C. D., Rathnamma D., Isloor, Shrikrishna, Veeregowda B. M., Sharada R., Shivashankar B. P., Sumathi B. R., Sudha G., Suryanarayan |
| المصدر: |
Indian Journal of Veterinary Sciences & Biotechnology; Nov/Dec2023, Vol. 19 Issue 6, p44-49, 6p |
| مصطلحات موضوعية: |
MYCOPLASMA, AMNIOTIC liquid, SYNOVIAL fluid, SHEEP, JOINTS (Anatomy), GOATS |
| مصطلحات جغرافية: |
KARNATAKA (India), INDIA |
| مستخلص: |
Mycoplasmosis is one of the economically important diseases in small ruminants. Mycoplasma affects respiratory system predominantly apart from synovial joints, udder and mucus membrane bearing surface. In the current study, a total of 514 samples consisting of 465 nasal swabs, 18 lung tissues, 16 milk, 11 synovial fluids, 3 ear swabs, and one fetal fluid were subjected for isolation of Mycoplasma in PPLO broth and PPLO agar. Inoculated samples were incubated at 37°C for 24-48 h under 5% CO2, until turbidity and colour change was observed. Broth cultures were also subjected for Mycoplasma genus specific and species specific PCR. Of the 514 samples that were cultured, 32 (6.22%) produced characteristic 'fried egg' microcolonies on the agar surface. Of the 32 isolates, 21 were M. ovipneumoniae, 7 were M. arginini and 4 unidentified mycoplasma species. Seventy five (14.59%) samples were positive for genus specific PCR. By species specific PCR, 21 (28%) samples were identified as M. ovipneumoniae, 13 (17.33%) M. arginini, whereas 41 (54.66) samples did not amplify with either of the clusters specific primers or other species specific primers and remain unidentified. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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