التفاصيل البيبلوغرافية
العنوان: |
Dissecting the Genetic Basis of the Technological, Functional, and Safety Characteristics of Lacticaseibacillus paracasei SRX10. |
المؤلفون: |
Kamarinou, Christina S., Kiousi, Despoina E., Repanas, Panagiotis, Argyri, Anthoula A., Chorianopoulos, Nikos G., Galanis, Alex |
المصدر: |
Microorganisms; Jan2024, Vol. 12 Issue 1, p93, 19p |
مصطلحات موضوعية: |
YOGURT, WHOLE genome sequencing, LACTIC acid bacteria, FETA cheese, COMPARATIVE genomics, LACTIC acid, DAIRY microbiology |
مستخلص: |
Nonstarter lactic acid bacteria (NSLAB) are major contributors to the unique characteristics (e.g., aroma, flavor, texture) of dairy and nondairy fermented products. Lc. paracasei SRX10 is an NSLAB strain originally isolated from a traditional Greek cheese and previously shown to exhibit favorable biotechnological characteristics. More specifically, the strain showed tolerance to simulated gastrointestinal conditions, exopolysaccharide (EPS) biosynthetic capacity, and lack of hemolytic activity and was used in the production of yoghurt and feta cheese with distinct organoleptic characteristics. The aim of the present study was to investigate these traits at the genome level through whole-genome sequencing (WGS), annotation, and comparative genomics. Functional annotation of the genome revealed that Lc. paracasei SRX10 can utilize different carbon sources, leading to the generation of flavor compounds, including lactic acid, acetate, ethanol, and acetoin. Similarly, full clusters for fatty acid biosynthesis, protein and peptide degradation, as well as genes related to survival under extreme temperatures, osmotic shock, and oxidative stress were annotated. Importantly, no transferable antibiotic resistance genes or virulence factors were identified. Finally, strain-specific primers based on genome-wide polymorphisms were designed for the efficient and rapid identification of Lc. paracasei SRX10 via multiplex PCR in fermented products. [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |