التفاصيل البيبلوغرافية
| العنوان: |
Karyotype Variability in Wild Narcissus poeticus L. Populations from Different Environmental Conditions in the Dinaric Alps †. |
| المؤلفون: |
Pustahija, Fatima, Bašić, Neđad, Siljak-Yakovlev, Sonja |
| المصدر: |
Plants (2223-7747); Jan2024, Vol. 13 Issue 2, p208, 15p |
| مصطلحات موضوعية: |
KARYOTYPES, POLYPLOIDY, GENOME size, CHROMOSOME polymorphism, FLUORESCENCE in situ hybridization, CHROMOSOMES, CHROMOSOMAL rearrangement |
| مصطلحات جغرافية: |
DINARIC Alps, BALKAN Peninsula |
| مستخلص: |
Narcissus poeticus L. (Amaryllidaceae), a facultative serpentinophyte, is a highly variable species and particularly important ancestor of cultivated daffodils, but is rarely studied in field populations. This study, based on natural populations in the Balkans, focused on karyotype variability, genome size, ploidy and the presence of B chromosomes. Thirteen native populations from different environmental and soil conditions were collected and analyzed using flow cytometry to estimate nuclear genome size, fluorescence in situ hybridization (FISH) for physical mapping of rDNA, fluorochrome labeling (chromomycin and Hoechst) for heterochromatin organization and silver nitrate staining of nucleoli for determining rRNA gene activity. The organization of rDNA and natural triploids is reported here for the first time. The presence of individuals with B chromosomes (in 9/13 populations) and chromosomal rearrangements was also detected. The observed B chromosome showed three different morphotypes. The most frequent submetacentric type showed four different patterns, mainly with active ribosomal genes. The results obtained show that N. poeticus has a dynamic genome with variable genome size due to the presence of polyploidy, B chromosomes and chromosomal rearrangements. It is hypothesized that the observed changes reflect the response of the genome to different environmental conditions, where individuals with B chromosomes appear to have certain adaptive advantages. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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