التفاصيل البيبلوغرافية
| العنوان: |
Investigation of mRNA Expressions of Kisspepeptin, Neurokinin B and Their Receptors in Mesenchymal Stem Cells Isolated from Human Placenta. |
| المؤلفون: |
Oksuz, Hale, Uckayabasi, Ali, Oksuz, Halil Ibrahim, Yilmaz, Mehmet Bertan |
| المصدر: |
Medical Records; 2024 Supplement, Vol. 6, p39-39, 2/3p |
| مصطلحات موضوعية: |
FLOW cytometry, KISSPEPTINS, CONFERENCES & conventions, GENE expression, MESSENGER RNA, PLACENTA, MESENCHYMAL stem cells |
| مستخلص: |
Aim: Kisspeptins and interacting GPR54 or KISS1R receptors are highly expressed in the placenta, pituitary, pancreas and medulla spinalis. Loss of KiSS-1 expression during cell degeneration has been associated with esophageal squamous cell carcinoma and bladder cancer. Therefore, it is important that kisspeptins and their receptors are functional, especially in patients who will use mesenchymal stem cells (MSCs) for a neurodegenerative disease. In our study, we investigated the presence of kisspeptin (KP) and neurokinin (NK) and aimed to compare their gene expression. Material and Methods: The MSCs used in our study were obtained from the chorionic membranes of the placenta samples of 5 surgically delivered babies, which were considered as medical waste, with the informed consent of the mothers. They were then analyzed by flow cell meter using surface markers and RNA isolation was performed. Expression of KP, NK and their receptors was analyzed by real-time polymerase chain reaction (RT-PCR). Results: Flow cytometry analysis of the cultured placenta tissue cells confirmed that they were MSCs. RT-PCR results showed that the expression of Kiss1 and Kiss1r mRNAs decreased depending on the number of passages. It was found that NK and NK receptor were not expressed in the placental MSCs. Conclusion: In our study, as the number of cell divisions and passages increased, there was a decrease in parental features and a decrease in the amount of KISS1 and KISS1R expression was observed. Our results will help future studies to determine the roles of KISS1 and GPR54 receptor interaction in neuroendocrinology. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
