التفاصيل البيبلوغرافية
| العنوان: |
bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration. |
| المؤلفون: |
Kawai, Mariko Yamamoto, Yoshida, Takeshi, Kato, Tomoki, Watanabe, Takuma, Kashiwagi, Marina, Yamanaka, Shigeki, Yamamoto, Hiromitsu, Nagahiro, Shigeki, Iwamoto, Tsutomu, Masud, Khan, Aoki, Kazuhiro, Ohura, Kiyoshi, Nakao, Kazumasa |
| المصدر: |
Materials (1996-1944); Feb2024, Vol. 17 Issue 4, p880, 9p |
| مصطلحات موضوعية: |
SKELETAL muscle, BIOMATERIALS, ELECTRODES, SOFT X rays, GENETIC transformation, BONE regeneration, GENETIC vectors |
| مستخلص: |
Background: Bone morphogenetic protein-2 (bmp-2) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the bmp-2 gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral bmp-2 gene expression plasmid vector (pCAGGS-bmp-2) and needle-type electrodes. Methods: We injected the bmp-2 gene with pCAGGS-bmp-2 into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the bmp-2 gene to skeletal muscles, making them potential biomaterials for repairing bone defects. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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