التفاصيل البيبلوغرافية
| العنوان: |
Molecular determination of Leptospira spp., street and shelters dogs from the Coffee Region of Colombia. |
| Alternate Title: |
Molekularno određivanje Leptospira spp. u uličnim psima i psima iz skloništa u kolumbijskoj Regiji kave. (Croatian) |
| المؤلفون: |
Caballero Méndez, L. C., González Londoño, L. M., Gómez Ruíz, J. C., Escobar Herrera, M. J., Mazo, M. M., Franco-Montoya, L. N. |
| المصدر: |
Veterinarska Stanica; 2024, Vol. 55 Issue 5, p527-534, 8p |
| مصطلحات موضوعية: |
LEPTOSPIRA, LEPTOSPIROSIS in animals, BLOOD sampling, POLYMERASE chain reaction |
| مصطلحات جغرافية: |
COLOMBIA |
| Abstract (English): |
Leptospirosis is considered a zoonotic disease with a substantial impact on animal and human health. It is distributed in tropical and subtropical climates, which improves the survival of these bacteria for a long time, affecting domestic and wild animals that act as a reservoir. Canine leptospirosis has obtained great clinical relevance, due to the susceptibility of this species to infection and the frequent exposure to leptospirosis from the environment. Canines from the street and animal shelters constitute a high-risk population due to the proximity to sources of infection. This paper describes a cross-sectional study that involves sampling dogs from shelters located in the departments of Risaralda, Valle del Cauca, and Caldas, known as the Coffee Region, located in the centre-west of Colombia. Blood samples were taken from 140 canines and analysed in the laboratory of the Veterinary Medicine and Animal Science, Technological University of Pereira. DNA was extracted, and the LipL32 gene was amplified by conventional PCR. A 15% prevalence for Leptospira sp. was found in dogs from the Coffee region. No correlation was found between the variables such as sex, age, origin, and socioeconomic status. However, a tendency for infection was observed with several cases of diagnosis in female dogs older than six years in low strata. This study constitutes the first report of canine leptospirosis in this region of Colombia, which will allow the design of strategies aiming to mitigate the disease in this region. [ABSTRACT FROM AUTHOR] |
| Abstract (Croatian): |
Leptospiroza se smatra zoonotskom bolešću i od značajnog je utjecaja na zdravlje ljudi i životinja. Prisutna je u tropskim i suptropskim klimama što potpomaže dugoročno preživljavanje ovih bakterija, utječući na domaće i divlje životinje koje su njihovi rezervoari. Leptospiroza pasa dobila je na velikom kliničkom značenju, između ostalog i zbog prijemčivosti ove vrste bakterija na infekciju i često izlaganje leptospirozi iz okoliša. Zbog blizine izvorima infekcije psi s ulice i iz skloništa za životinje predstavljaju visokorizičnu opasnost. U ovom radu opisana je unakrsnu studija koja uključuje uzorkovanje pasa iz skloništa koja se nalaze u departmanima Risaralda, Valle del Cauca i Caldas, poznatima kao Regija kave, u srednjem zapadu Kolumbije. Uzeti su uzorci krvi Molekularno određivanje Leptospira spp. u uličnim psima i psima iz skloništa u kolumbijskoj Regiji kave Lyda C. CABALLERO MÉNDEZ, Grupo de investigación BIOECOS, Profesora de Medicina Veterinaria y Zootecnia Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia; Luisa María GONZÁLEZ LONDOÑO, Julio Cesar GÓMEZ RUÍZ, María José ESCOBAR HERRERA, Médico Veterinaria y Zootecnista. Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia; Margarita María MAZO, Grupo de investigación BIOECOS, Profesora de Medicina Veterinaria y Zootecnia Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira, Colombia, Luz Natalia FRANCOMONTOYA, Grupo de investigación CIBAV, Profesora Asociada adscrita a la Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad de Antioquia UdeA, Medellín, Colombia 140 pasa, analizirani su u laboratoriju za Veterinarsku medicinu i znanost o životinjama Tehnološkog Sveučilišta u Pereiri, ekstrahirana je DNK te je amplificiran LipL32 gen konvencionalnim PCR-om. Dokazana je prevalencija od 15 % za Leptospira sp. u pasa iz Regije kave; nije otkrivena korelacija između varijabli poput spola, dobi, podrijetla i socioekonomskog statusa. Međutim, zamijećena je tendencija za infekciju s nekoliko slučajeva dijagnoze u kuja starijih od šest godina u nižim slojevima. Ova studija predstavlja prvo izviješće o leptospirozi pasa u ovoj regiji u Kolumbiji, što će omogućiti osmišljavanje strategija usmjerenih na ublažavanje ove bolesti. [ABSTRACT FROM AUTHOR] |
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