التفاصيل البيبلوغرافية
| العنوان: |
彝药斯赤列甲醇提取物对骨关节炎模型大鼠的治疗作用及靶点预测. (Chinese) |
| Alternate Title: |
Yi medicine Sambucus adnata Wall methanol extract in a rat osteoarthritis model: therapeutic effect and target prediction. (English) |
| المؤلفون: |
郑俭彬, 陆玉春, 江自鲜, 李秀芳, 王 涛, 王文静 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 8/18/2024, Vol. 28 Issue 23, p3627-3635, 9p |
| Abstract (English): |
BACKGROUND: The Yi people have used Sambucus adnata Wall to treat fractures, bruises, arthritis, etc. The author’s group found that the active site aqueous extract (SAW-A) and dichloromethane extract (SAW-B) can promote fracture healing by promoting the expression of genes related to osteoblast proliferation, differentiation and maturation, differentiation and maturation. However, the therapeutic effect of methanol extract (SAW-C) at its active site on osteoarthritis is unclear. OBJECTIVE: To investigate the therapeutic effect of Sambucus adnata Wall extract on osteoarthritis, and to identify the effective targets of Sambucus adnata Wall extract in the treatment of osteoarthritis by network pharmacology technology. METHODS: A rat osteoarthritis model was replicated by anterior cruciate ligamentectomy and model rats were then treated with methanol extract of Sambucus adnata Wall by gavage for 21 days. On the 21st day after modeling, the knee joint of rats was detected by X-ray, the width of the knee joint was measured, oxidative stress indexes and inflammatory factors levels in serum and joint lavage fluid were detected, the morphology of the knee joint was observed by hematoxylin-eosin staining, full-thickness cartilage and hyaline cartilage thickness were measured, and the content of articular cartilage proteoglycan was observed by safranin O-fast green staining. The "drug-component-disease-target" network was constructed. Gene ontology enrichment analysis and Kyoto encyclopedia of genes and genomes enrichment analysis of effective targets were performed, and protein-protein interaction network maps were constructed using cytoscape software. RESULTS AND CONCLUSION: Sambucus adnata Wall extract could reduce tumor necrosis factor-α level in joint lavage fluid and serum levels of prostaglandin E2 and malondialdehyde, while increase the level of superoxide dismutase; relieve joint swelling caused by osteoarthritis; improve the histopathological state of articular cartilage, maintain the thickness of full-thickness articular cartilage, hyaline cartilage and the area of bone trabeculae in subchondral bone; and effectively prevent the loss of proteoglycans in articular cartilage and have a chondroprotective effect. Network pharmacological results showed that the methanol extract of Sambucus adnata Wall may have some targets related to inhibition of tumor necrosis factor, AKT1, interleukin-6, MAPK3, and SRC as well as inhibition of over-activation of EGFR signaling pathway and AGE-RAGE signaling pathway. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：彝族人民将斯赤列用于治疗骨折、跌打劳伤、关节炎等。作者所在课题组前期研究发现斯赤列活性部位水提取物(SAW-A)和二氯甲 烷提取物(SAW-B)可通过促进成骨细胞增殖、分化和成熟相关基因的表达发挥促进骨折愈合作用，但其活性部位甲醇提取物(SAW-C)对骨关 节炎的治疗作用尚不清楚。 目的：探讨斯赤列甲醇提取物对骨关节炎的治疗作用，并通过网络药理学技术筛选斯赤列甲醇提取物治疗骨关节炎的有效靶点。 方法：通过大鼠前交叉韧带切断手术复制大鼠骨关节炎模型，术后连续灌胃斯赤列甲醇总提物21 d，于造模第21天进行X射线影像学观 察，测量膝关节宽度，检测血清中氧化应激指标和关节腔灌洗液中炎症因子水平，苏木精-伊红染色病理学观察，测量全层软骨厚度和透 明软骨厚度，番红-固绿染色观察关节软骨蛋白聚糖含量。构建“药物-成分-疾病-靶点”网络，对有效靶点进行GO富集分析和KEGG富集 分析，运用cytoscape软件构建蛋白互作网络图。 结果与结论：①斯赤列甲醇提取物可降低骨关节炎大鼠关节腔灌洗液中肿瘤坏死因子α与血清中前列腺素E2、丙二醛水平，提高超氧化物 歧化酶水平；缓解由骨关节炎所致的关节肿胀；改善关节软骨组织病理状态，维持关节全层软骨、透明软骨厚度以及软骨下骨中骨小梁 面积；防止关节软骨中蛋白聚糖的流失，对软骨有保护作用；②网络药理学结果表明，斯赤列甲醇提取物的作用靶点可能与抑制TNF、 AKT1、IL-6、MAPK3、SRC等因子释放，以及抑制EGFR信号通路和AGE-RAGE信号通路的过度激活有关。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
