التفاصيل البيبلوغرافية
| العنوان: |
A statistical approach to system suitability testing for mass spectrometry imaging. |
| المؤلفون: |
Sohn, Alexandria L., Kibbe, Russell R., Dioli, Olivia E., Hector, Emily C., Bai, Hongxia, Garrard, Kenneth P., Muddiman, David C. |
| المصدر: |
Rapid Communications in Mass Spectrometry: RCM; May2024, Vol. 38 Issue 9, p1-10, 10p |
| مصطلحات موضوعية: |
MASS spectrometry, DESORPTION electrospray ionization, TEST systems, INFRARED lasers, PRINCIPAL components analysis |
| مستخلص: |
Rationale: Mass spectrometry imaging (MSI) elevates the power of conventional mass spectrometry (MS) to multidimensional space, elucidating both chemical composition and localization. However, the field lacks any robust quality control (QC) and/or system suitability testing (SST) protocols to monitor inconsistencies during data acquisition, both of which are integral to ensure the validity of experimental results. To satisfy this demand in the community, we propose an adaptable QC/SST approach with five analyte options amendable to various ionization MSI platforms (e.g., desorption electrospray ionization, matrix‐assisted laser desorption/ionization [MALDI], MALDI‐2, and infrared matrix‐assisted laser desorption electrospray ionization [IR‐MALDESI]). Methods: A novel QC mix was sprayed across glass slides to collect QC/SST regions‐of‐interest (ROIs). Data were collected under optimal conditions and on a compromised instrument to construct and refine the principal component analysis (PCA) model in R. Metrics, including mass measurement accuracy and spectral accuracy, were evaluated, yielding an individual suitability score for each compound. The average of these scores is utilized to inform if troubleshooting is necessary. Results: The PCA‐based SST model was applied to data collected when the instrument was compromised. The resultant SST scores were used to determine a statistically significant threshold, which was defined as 0.93 for IR‐MALDESI‐MSI analyses. This minimizes the type‐I error rate, where the QC/SST would report the platform to be in working condition when cleaning is actually necessary. Further, data scored after a partial cleaning demonstrate the importance of QC and frequent full instrument cleaning. Conclusions: This study is the starting point for addressing an important issue and will undergo future development to improve the efficiency of the protocol. Ultimately, this work is the first of its kind and proposes this approach as a proof of concept to develop and implement universal QC/SST protocols for a variety of MSI platforms. [ABSTRACT FROM AUTHOR] |
|
Copyright of Rapid Communications in Mass Spectrometry: RCM is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder