التفاصيل البيبلوغرافية
| العنوان: |
Anti‐Candida activity and in vitro toxicity screening of antifungals complexed with β‐cyclodextrin. |
| المؤلفون: |
Moraes, Gustavo Simão, Tozetto, Nathaly Mayer, Pedroso, Thaynara Aparecida Alves, de Mattos, Marcela Alves, Urban, Amanda Migliorini, Paludo, Katia Sabrina, dos Santos, Fábio André, Neppelenbroek, Karin Hermana, Urban, Vanessa Migliorini |
| المصدر: |
Journal of Applied Toxicology; May2024, Vol. 44 Issue 5, p747-755, 9p |
| مصطلحات موضوعية: |
CYCLODEXTRINS, CONTROLLED release drugs, ONE-way analysis of variance, ANTIFUNGAL agents, ANTI-infective agents, PROBIT analysis |
| مستخلص: |
The emergence of resistant fungal species and the toxicity of currently available antifungal drugs are relevant issues that require special consideration. Cyclodextrins inclusion complexes could optimize the antimicrobial activity of such drugs and create a controlled release system with few side effects. This study aimed to assess the in vitro toxicity and antifungal effectiveness of nystatin (Nys) and chlorhexidine (Chx) complexed or not with β‐cyclodextrin (βCD). First, a drug toxicity screening was performed through the Artemia salina bioassay. Then, the minimum inhibitory concentrations (MICs) against Candida albicans were determined with the broth microdilution test. After MICs determination, the cytotoxicity of the drugs was evaluated through the methyl‐thiazolyl‐tetrazolium (MTT) and neutral red (NR) assays and through cell morphology analysis. The PROBIT analysis was used to determine the median lethal concentration (LC50), and the cell viability values were submitted to one‐way analysis of variance(ANOVA)/Tukey (α = 0.05). Overall, the βCD‐complexed antifungals were less toxic against A. salina than their raw forms, suggesting that inclusion complexes can reduce the toxicity of drugs. The MICs obtained were as follows: Nys 0.5 mg/L; Nys:βCD 4 mg/L; Chx 4 mg/L; and Chx:βCD 8 mg/L. Chx showed significant cytotoxicity (MTT: 12.9 ± 9.6%; NR: 10.6 ± 12.5%) and promoted important morphological changes. Cells exposed to the other drugs showed viability above 70% with no cellular damage. These results suggest that antifungals complexed with βCD might be a biocompatible option for the treatment of Candida‐related infections. Our study aimed to evaluate the in vitro toxicity of nystatin and chlorhexidine complexed with β‐cyclodextrin (βCD) against Artemia salina, to determine their minimum inhibitory concentrations against Candida albicans, and to assess their cytotoxicity on murine fibroblasts at these concentrations. The complexed antifungals were less toxic to A. salina than their raw forms. βCD‐complexed drugs also showed excellent antifungal activity against C. albicans and did not promote cellular damage. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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