التفاصيل البيبلوغرافية
| العنوان: |
Simultaneous determination of five plant hormones in cotton leaves using QuEChERS combined with HPLC‒MS/MS. |
| المؤلفون: |
Wang, Wenhao, Song, Xianpeng, Wang, Dan, Ma, Yajie, Shan, Yongpan, Ren, Xiangliang, Hu, Hongyan, Wu, Changcai, Yang, Jun, Ma, Yan |
| المصدر: |
Journal of Cotton Research; 5/13/2024, Vol. 7 Issue 1, p1-14, 14p |
| مصطلحات موضوعية: |
PLANT hormones, COTTON, COTTON trade, REGRESSION analysis, PHYSIOLOGY |
| مستخلص: |
Background: Plant hormones profoundly influence cotton growth, development, and responses to various stresses. Therefore, there is a pressing need for an efficient assay to quantify these hormones in cotton. In this groundbreaking study, we have established QuEChERS-HPLC‒MS/MS method, for the simultaneous detection of multiple plant hormones in cotton leaves, allowing the analysis and quantification of five key plant hormones. Results: Sample extraction and purification employed 0.1% acetic acid in methanol and C18 for optimal recovery of plant hormones. The method applied to cotton demonstrated excellent linearity across a concentration range of 0.05–1 mg·L−1, with linear regression coefficients exceeding 0.99. The limits of quantification (LOQs) were 20 μg·kg−1 for GA3 and 5 μg·kg−1 for the other four plant hormones. Recovery rates for the five plant hormones matrix spiked at levels of 5, 10, 100, and 1000 μg·kg−1 were in the range of 79.07% to 98.97%, with intraday relative standard deviations (RSDs) ranging from 2.11% to 8.47%. The method was successfully employed to analyze and quantify the five analytes in cotton leaves treated with plant growth regulators. Conclusion: The study demonstrates that the method is well-suited for the determination of five plant hormones in cotton. It exhibits excellent selectivity and sensitivity in detecting field samples, thus serving as a robust tool for in-depth research into cotton physiology. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Cotton Research is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Find this article in full text from Springer Verlag. 
Full Text Finder