التفاصيل البيبلوغرافية
العنوان: |
Simultaneous determination of five plant hormones in cotton leaves using QuEChERS combined with HPLC‒MS/MS. |
المؤلفون: |
Wang, Wenhao, Song, Xianpeng, Wang, Dan, Ma, Yajie, Shan, Yongpan, Ren, Xiangliang, Hu, Hongyan, Wu, Changcai, Yang, Jun, Ma, Yan |
المصدر: |
Journal of Cotton Research; 5/13/2024, Vol. 7 Issue 1, p1-14, 14p |
مصطلحات موضوعية: |
PLANT hormones, COTTON, COTTON trade, REGRESSION analysis, PHYSIOLOGY |
مستخلص: |
Background: Plant hormones profoundly influence cotton growth, development, and responses to various stresses. Therefore, there is a pressing need for an efficient assay to quantify these hormones in cotton. In this groundbreaking study, we have established QuEChERS-HPLC‒MS/MS method, for the simultaneous detection of multiple plant hormones in cotton leaves, allowing the analysis and quantification of five key plant hormones. Results: Sample extraction and purification employed 0.1% acetic acid in methanol and C18 for optimal recovery of plant hormones. The method applied to cotton demonstrated excellent linearity across a concentration range of 0.05–1 mg·L−1, with linear regression coefficients exceeding 0.99. The limits of quantification (LOQs) were 20 μg·kg−1 for GA3 and 5 μg·kg−1 for the other four plant hormones. Recovery rates for the five plant hormones matrix spiked at levels of 5, 10, 100, and 1000 μg·kg−1 were in the range of 79.07% to 98.97%, with intraday relative standard deviations (RSDs) ranging from 2.11% to 8.47%. The method was successfully employed to analyze and quantify the five analytes in cotton leaves treated with plant growth regulators. Conclusion: The study demonstrates that the method is well-suited for the determination of five plant hormones in cotton. It exhibits excellent selectivity and sensitivity in detecting field samples, thus serving as a robust tool for in-depth research into cotton physiology. [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |