التفاصيل البيبلوغرافية
| العنوان: |
The neuropeptide pigment‐dispersing factor signals independently of Bruchpilot‐labelled active zones in daily remodelled terminals of Drosophila clock neurons. |
| المؤلفون: |
Hofbauer, Benedikt, Zandawala, Meet, Reinhard, Nils, Rieger, Dirk, Werner, Christian, Evers, Jan Felix, Wegener, Christian |
| المصدر: |
European Journal of Neuroscience; May2024, Vol. 59 Issue 10, p2665-2685, 21p |
| مصطلحات موضوعية: |
DROSOPHILA, EXPANSION microscopy, NEURONS, SUPRACHIASMATIC nucleus |
| مستخلص: |
The small ventrolateral neurons (sLNvs) are key components of the central clock in the Drosophila brain. They signal via the neuropeptide pigment‐dispersing factor (PDF) to align the molecular clockwork of different central clock neurons and to modulate downstream circuits. The dorsal terminals of the sLNvs undergo daily morphological changes that affect presynaptic sites organised by the active zone protein Bruchpilot (BRP), a homolog of mammalian ELKS proteins. However, the role of these presynaptic sites for PDF release is ill‐defined. Here, we combined expansion microscopy with labelling of active zones by endogenously tagged BRP to examine the spatial correlation between PDF‐containing dense‐core vesicles and BRP‐labelled active zones. We found that the number of BRP‐labelled puncta in the sLNv terminals was similar while their density differed between Zeitgeber time (ZT) 2 and 14. The relative distance between BRP‐ and PDF‐labelled puncta was increased in the morning, around the reported time of PDF release. Spontaneous dense‐core vesicle release profiles of sLNvs in a publicly available ssTEM dataset (FAFB) consistently lacked spatial correlation to BRP‐organised active zones. RNAi‐mediated downregulation of brp and other active zone proteins expressed by the sLNvs did not affect PDF‐dependent locomotor rhythmicity. In contrast, down‐regulation of genes encoding proteins of the canonical vesicle release machinery, the dense‐core vesicle‐related protein CADPS, as well as PDF impaired locomotor rhythmicity. Taken together, our study suggests that PDF release from the sLNvs is independent of BRP‐organised active zones, while BRP may be redistributed to active zones in a time‐dependent manner. [ABSTRACT FROM AUTHOR] |
|
Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder