التفاصيل البيبلوغرافية
| العنوان: |
Development of a Single-Step SYBR Green based Real-Time PCR Assay for Identification and Quantification of Fowl Pox Virus in Chicken. |
| المؤلفون: |
Sanganagouda K., Kounin, Sabha, Nagraja K., Sajjanar, Basavaraj, Gomes, Amitha Rena, Sahoo, Aditya Prasad, Alamelu M. S. |
| المصدر: |
Indian Journal of Veterinary Sciences & Biotechnology; 2024, Vol. 20 Issue 3, p15-19, 5p |
| مصطلحات موضوعية: |
VARICELLA-zoster virus, H7N9 Influenza, POULTRY farm management, POULTRY, POXVIRUSES, FOWLING |
| مستخلص: |
Fowlpox is a re-emerging and economically significant viral disease in poultry. Fowlpox is a complex virus contains a double-strand DNA genome of approximately 300 kb and largest among all pox viruses. In the present study, a clinical case of fowlpox virus (FPV) infection was investigated and a Real-Time PCR (RT-PCR) assay was developed for confirmatory diagnosis of fowl pox and quantification of viral load. Clinical sample was collected from infected bird reared in the organized poultry farm management system and the viral infection was confirmed using conventional PCR amplification of the P4b gene of fowl pox virus. Following that, a single-step SYBR green based Real-Time PCR assay was developed by targeting the same gene of fowlpox virus. The fowl pox viral load in the infected bird was found to be 7.33 ± 0.044 (Log Mean ± SD). The lower detection limit achieved was 134 copies per microliter (µL) with a corresponding threshold cycle (Ct) value of 32.59. Thus, the single-step SYBR green based RT-PCR assay targeting the P4b gene of fowl pox virus reported in this study was highly sensitive, specific and reproducible for the detection and quantitation of fowl pox virus nucleic acids. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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