| Abstract (English): |
【Objective】 To analyze the composition and content of maize leaf volatile before and after the damage caused by Monolepta signata in the hope of providing a scientific basis for further exploring the defense mechanism of maize leaf volatile against M. signata.【Methods】 In this study, the volatile compounds of maize leaves and M.signata were detected and analyzed by GC-MS at different feeding induction time(6, 24, 36 and 48 h).【Results】 The results showed that 29, 30, 33, 36 and 26 volatile substances, mainly including aldehydes, terpenoids, alcohols and ketones, were detected after 0, 6, 24, 36 and 48 h of feeding induction by the M. signata respectively. Twenty volatiles, including 1-stigmasterene, cis-11-hexadecanal, α-stigmasterene and hexanal, disappeared with the insect after feeding on the damage; 42 compounds, such as 2-hexenal, (E)-2-hexenal, α-pinene and β-laurene, were newly collected. The results of principal component analysis showed that α-pinene, 2-hexenal and β-pinene were the main compounds released under 6h and 24h of infestation; hexadecanal, tetradecanal, n-pentadecanal, β-pirolone and(E)-3-hexen-1-ol were the main compounds released at 36 h of infestation; β-red myrcene alcohol, nerolidol, (1R) -α-pinene, β-rolene, β-sandalene β-cineole, and humulene were the main compounds released at 48 h of insect infestation.【Conclusion】 The composition and content of volatile compounds in maize leaves at different feeding times have a significant impact on the M. signata. Under 6 and 24 hours of pest infestation, it mainly releases olefins, while at 36 hours of pest infestation, it mainly releases aldehydes and ketones. After 48 hours of pest infestation, it mainly releases alcohols and olefins. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
【目的】分析双斑长跗萤叶甲取食前后的玉米叶片挥发物组成和含量, 为进一步研究玉米叶片挥发物对双斑萤叶甲的防御机制提供科学依据。【方法】采用固相微萃取和气相色谱-质谱联用仪检测并分析健康玉米叶片及双斑长跗萤叶甲不同取食时间 (6、24、36 和 48 h)下玉米叶片挥发物组分变化。【结果】双斑长跗萤叶甲取食 0、6、24、36 和 48 h 后, 分别检测到 29、30、33、36、26 种挥发物, 主要为醛类、萜类、醇类及酮类等化合物。1-石竹烯、顺-11-十六醛、α-石竹烯、正己醛等20种挥发物随该虫取食为害后消失;新收集到2-己烯醛、(E)-2-己烯醛、α-蒎烯、β-月桂烯等42种化合物。α-蒎烯、2-己烯醛、β-蒎烯等物质是虫害 6 及 24 h 释放的主要化合物; 十六醛、十四醛、正十五碳醛、β-紫罗酮、(E)-3- 己烯 -1- 醇是虫害 36 h 释放的主要化合物; β-红没药醇、橙花叔醇、(1R)-α-蒎烯、β-罗勒烯、β-檀香烯、β-月桂烯、葎草烯是虫害48 h释放的主要化合物。【结论】双斑长跗萤叶甲不同时间取食玉米叶片的挥发物的组分和含量影响较大, 取食 6 及 24 h 主要释放烯烃类化合物, 取食 36 h 主要释放醛类和酮类化合物, 取食 48 h 主要释放醇类、烯烃类化合物。 [ABSTRACT FROM AUTHOR] |
