التفاصيل البيبلوغرافية
| العنوان: |
Decreased NMIIA heavy chain phosphorylation at S1943 promotes mitoxantrone resistance by upregulating BCRP and N-cadherin expression in breast cancer cells. |
| المؤلفون: |
Li, Kemin, Li, Tian, Niu, Yanan, Gao, Yu, Shi, Yifan, He, Yifan, Zhang, Xuanping, Wang, Yan, Cao, Jing, Hu, Xiaoling, Chen, Min, Shi, Ruizan |
| المصدر: |
Biochemistry & Cell Biology; 2024, Vol. 102 Issue 3, p213-225, 13p |
| مصطلحات موضوعية: |
BREAST cancer, CADHERINS, CANCER cells, EPIDERMAL growth factor, PHOSPHORYLATION, CATENINS, BREAST |
| مستخلص: |
Mitoxantrone (MX) is an effective treatment for breast cancer; however, high efflux of MX that is accomplished by breast cancer resistance protein (BCRP) leads to acquired multidrug resistance (MDR), reducing MX's therapeutic efficacy in breast cancer. Non-muscle myosin IIA (NMIIA) and its heavy phosphorylation at S1943 have been revealed to play key roles in tumor metastasis and progression, including in breast cancer; however, their molecular function in BCRP-mediated MDR in breast cancer remains unknown. In this study, we revealed that the expression of NMIIA heavy chain phosphorylation at S1943 was downregulated in BCRP-overexpressing breast cancer MCF‐7/MX cells, and stable expression of NMIIA-S1943A mutant increased BCRP expression and promoted the resistance of MCF‐7/MX cells to MX. Meanwhile, NMIIA S1943 phosphorylation induced by epidermal growth factor (EGF) was accompanied by the downregulation of BCRP in MCF-7/MX cells. Furthermore, stable expression of NMIIA-S1943A in MCF-7/MX cells resulted in upregulation of N-cadherin and the accumulation of β-catenin on the cell surface, which inhibited the nucleus translocation of β-catenin and Wnt/β-catenin-based proliferative signaling. EGF stimulation of MCF-7/MX cells showed the downregulation of N-cadherin and β-catenin. Our results suggest that decreased NMIIA heavy phosphorylation at S1943 increases BCRP expression and promotes MX resistance in breast cancer cells via upregulating N-cadherin expression. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
