التفاصيل البيبلوغرافية
| العنوان: |
Stochastic Optical Reconstruction Microscopy Imaging of Multiple System Atrophy Inclusions Suggests Stepwise α-Synuclein Aggregation. |
| المؤلفون: |
Vovard, Benoît, Bodin, Alexia, Gouju, Julien, de Guilhem de Lataillade, Adrien, Derkinderen, Pascal, Etcharry-Bouyx, Frédérique, Chauviré, Valérie, Guillet-Pichon, Virginie, Verny, Christophe, Letournel, Franck, Lenaers, Guy, Chevrollier, Arnaud, Codron, Philippe |
| المصدر: |
Movement Disorders; Apr2024, Vol. 39 Issue 4, p723-728, 6p |
| مستخلص: |
Background: The architecture and composition of glial (GCI) and neuronal (NCI) α-synuclein inclusions observed in multiple system atrophy (MSA) remain to be precisely defined to better understand the disease. Methods: Here, we used stochastic optical reconstruction microscopy (STORM) to characterize the nanoscale organization of glial (GCI) and neuronal (NCI) α-synuclein inclusions in cryopreserved brain sections from MSA patients. Results: STORM revealed a dense cross-linked internal structure of α-synuclein in all GCI and NCI. The internal architecture of hyperphosphorylated α-synuclein (p-αSyn) inclusions was similar in glial and neuronal cells, suggesting a common aggregation mechanism. A similar sequence of p-αSyn stepwise intracellular aggregation was defined in oligodendrocytes and neurons, starting from the perinuclear area and growing inside the cells. Consistent with this hypothesis, we found a higher mitochondrial density in GCI and NCI compared to oligodendrocytes and neurons from unaffected donors (P < 0.01), suggesting an active recruitment of the organelles during the aggregation process. Conclusions: These first STORM images of GCI and NCI suggest stepwise α-synuclein aggregation in MSA. © 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. [ABSTRACT FROM AUTHOR] |
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