التفاصيل البيبلوغرافية
| العنوان: |
Expediting Next-Generation Hybrid Technology in Recalcitrant Maize Inbreds through In Vivo Targeted Activity of CRISPR/Cas9. |
| المؤلفون: |
Hou, Liudi, Xiao, Bing, Zhu, Jinjie, Liu, Changlin, Wu, Qingyu, Xie, Chuanxiao, Zou, Huawen, Qi, Xiantao |
| المصدر: |
International Journal of Molecular Sciences; Jun2024, Vol. 25 Issue 11, p5832, 15p |
| مصطلحات موضوعية: |
CRISPRS, SEED technology, CORN breeding, SEED industry, INBREEDING, CORN |
| مستخلص: |
The Manipulated Genic Male Sterile Maintainer (MGM) system, a next-generation hybrid seed technology, enables efficient production of sortable seeds from genic male sterile (GMS) lines. However, implementing robust MGM systems in commercial maize inbred lines requires stable transformation, a genotype-specific and laborious process. This study aimed to integrate MGM technology into the commercial maize inbred line Z372, developing both GMS and MGM lines. We utilized the MGM line ZC01-3A-7, which contains the MS26ΔE5 editor T-DNA and MGM T-DNA, previously established in the highly transformable ZC01 recipient plants. Through a combination of crossing and backcrossing with Z372, we targeted the fertility gene Ms26 within the Z372 genome for mutation using the in vivo CRISPR/Cas9 activity within the MS26ΔE5 editor T-DNA construct. This approach facilitated precise editing of the Ms26 locus, minimizing linkage drag associated with the Ms26 mutation. Whole-genome SNP analysis achieved a 98.74% recovery rate for GMS and 96.32% for MGM in the BC2F2 generation. Importantly, the Z372-GMS line with the ms26ΔE5 mutation is non-transgenic, avoiding linkage drag and demonstrating production readiness. This study represents a significant advancement in maize breeding, enabling the rapid generation of GMS and MGM lines for efficient hybrid seed production. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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