التفاصيل البيبلوغرافية
| العنوان: |
Radiolabeled iron oxide nanoparticles functionalized with PSMA/BN ligands for dual-targeting of prostate cancer. |
| المؤلفون: |
Bajwa, Danae Efremia, Salvanou, Evangelia-Alexandra, Theodosiou, Maria, Koutsikou, Theodora S., Efthimiadou, Eleni K., Bouziotis, Penelope, Liolios, Christos |
| المصدر: |
Frontiers in Nuclear Medicine; 2024, p1-16, 16p |
| مصطلحات موضوعية: |
IN vitro studies, WOUND healing, LIGANDS (Chemistry), SINGLE-photon emission computed tomography, RESEARCH funding, PROSTATE tumors, RADIOISOTOPES, TECHNETIUM, PEPTIDE hormones, DESCRIPTIVE statistics, IRON compounds, PROSTATE-specific membrane antigen, MOLECULAR structure, ANALYSIS of variance, HEMOLYSIS & hemolysins, BIOLOGICAL assay, COMPARATIVE studies, NANOPARTICLES, CELL receptors |
| مستخلص: |
Introduction: Prostate cancer (PCa) is the second most frequent cancer diagnosis in men and the fifth leading cause of death worldwide. Prostate Specific Membrane Antigen (PSMA) and Gastrin Releasing Peptide (GRP) receptors are overexpressed in PCa. In this study, we have developed iron oxide nanoparticles (IONs) functionalized with the Prostate Specific Membrane Antigen (PSMA) and Gastrin Releasing Peptide (GRP) ligands for dual targeting of Prostate cancer. Methods: IONs were developed with a thin silica layer on their surface with MPTES (carrying -SH groups, IONs-SH), and they were coupled either with a pharmacophore targeting PSMA (IONs-PSMA) or with bombesin peptide (IONs-BN), targeting GRP receptors, or with both (IONs-PSMA/BN). The functionalized IONs were characterized for their size, zeta potential, and efficiency of functionalization using dynamic light scattering (DLS) and Fourier-Transform Infrared Spectroscopy (FT-IR). All the aforementioned types of IONs were radiolabeled directly with Technetium-99m (99mTc) and evaluated for their radiolabeling efficiency, stability, and binding ability on two different PCa cell lines (PC3 and LNCaP). Results and Discussion: The MTT assay demonstrated low toxicity of the IONs against PC3 and LNCaP cells, while the performed wound-healing assay further proved that these nanostructures did not affect cellular growth mechanisms. The observed hemolysis ratio after co-incubation with red blood cells was extremely low. Furthermore, the 99mTc-radiolabeled IONs showed good stability in human serum, DTPA, and histidine, and high specific binding rates in cancer cells, supporting their future utilization as potential diagnostic tools for PCa with Single Photon Emission Computed Tomography (SPECT) imaging. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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