التفاصيل البيبلوغرافية
| العنوان: |
Mechanisms of Triton X-100 reducing the Ag+-resistance of Enterococcus faecalis. |
| المؤلفون: |
Lv, Silei, Duan, Mengting, Fan, Bing, Fan, Wei |
| المصدر: |
World Journal of Microbiology & Biotechnology; Jul2024, Vol. 40 Issue 7, p1-11, 11p |
| مصطلحات موضوعية: |
TRITON X-100, ENTEROCOCCUS, ENTEROCOCCUS faecalis, MEMBRANE potential, TRANSMISSION electron microscopy, MEMBRANE permeability (Biology) |
| مستخلص: |
To investigate the mechanism of Triton X-100 (TX-100) reducing the Ag+-resistance of Enterococcus faecalis (E. faecalis), and evaluate the antibacterial effect of TX-100 + Ag+ against the induced Ag+-resistant E. faecalis (AREf). The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of AgNO3 against E. faecalis with/without TX-100 were determined to verify the enhanced antibacterial activity. Transmission electron microscopy (TEM) was used to observe the morphological changes of E. faecalis after treatment. The intra- and extracellular concentration of Ag+ in treated E. faecalis was evaluated using inductively coupled plasma mass spectrometer (ICP-MS). The changes in cell membrane potential and integrity of treated E. faecalis were also observed using the flow cytometer. Moreover, AREf was induced through continuous exposure to sub-MIC of Ag+ and the antibacterial effect of TX-100 + Ag+ on AREf was further evaluated. The addition of 0.04% TX-100 showed maximal enhanced antibacterial effect of Ag+ against E. faecalis. The TEM and ICP-MS results demonstrated that TX-100 could facilitate Ag+ to enter E. faecalis through changing the membrane structure and integrity. Flow cytometry further showed the effect of TX-100 on membrane potential and permeability of E. faecalis. In addition, the enhanced antibacterial effect of TX-100 + Ag+ was also confirmed on induced AREf. TX-100 can facilitate Ag+ to enter E. faecalis through disrupting the membrane structure and changing the membrane potential and permeability, thus reducing the Ag+-resistance of E. faecalis and enhancing the antibacterial effect against either normal E. faecalis or induced AREf. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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