التفاصيل البيبلوغرافية
| العنوان: |
A Histological and Biomechanical Analysis of Human Acellular Dermis (HAD) Created Using a Novel Processing and Preservation Technique. |
| المؤلفون: |
Shah, Damini, Rathod, Madhu, Tiwari, Anjali, Kini, Abhishek, Bhagunde, Prasad, Bagaria, Vaibhav |
| المصدر: |
Indian Journal of Orthopaedics; Jul2024, Vol. 58 Issue 7, p922-931, 10p |
| مصطلحات موضوعية: |
PREVENTION of infectious disease transmission, DERMIS, IN vitro studies, BIOMECHANICS, TRANSPLANTATION of organs, tissues, etc., FREEZE-drying, TISSUE engineering, MEDICAL cadavers, GLYCERIN, HOMOGRAFTS, PRESERVATION of organs, tissues, etc., DESCRIPTIVE statistics, STERILIZATION (Disinfection), GRAFT rejection, TENSILE strength, COLLECTION & preservation of biological specimens, STAINS & staining (Microscopy) |
| مستخلص: |
Background: Large and complex defects requiring reconstruction are challenging for orthopaedic surgeons. The use of human acellular dermal (HAD) matrices to augment large soft tissue defects such as those seen in massive rotator cuff tears, knee extensor mechanism failures and neglected Tendo-Achilles tears has proven to be a valuable tool in surgeons reconstructive armamentarium. Different methods for allograft decellularization and preservation alter the native properties of the scaffold. Traditional processing and preservation methods have shown to have drawbacks that preclude its widespread use. Some of the common issues include inferior biomechanical properties, the risk of rejection, limited customization, difficulty in storing and transporting, the requirement of pre-operative preparation, and last but not the least increased cost. Methods: We describe a novel processing and preservation method utilizing a two-step non-denaturing decellularization method coupled with preservation using a water-sequestering agent (glycerol) to remove immunogenic components while retaining biomechanical properties. The efficiency of this novel process was compared with the traditional freeze-drying method and verified by histological evaluation and biomechanical strength analysis. Results: The absence of cellular components and matrix integrity in hematoxylin and eosin-stained glycerol-preserved HAD (gly-HAD) samples compared to freeze-dried HAD (FD-HAD) demonstrated effective yet gentle decellularization. Biomechanical strength analysis revealed that gly-HADs are stronger with an ultimate tensile load to the failure strength of 210 N compared to FD-HAD (124N). The gly-HADs were found to have an optimal suture–retention strength of 126 N. Finally, sterility testing of the resultant grafts was checked to ensure a sterility assurance level of 10−6 to establish implantability. Conclusion: The novel processing and preservation technique is described in this paper to create a Human Acellular Dermis with higher biomechanical strength and superior histological characteristics. The processing and preservation technique ensured high sterility assurance levels to establish implantability. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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