التفاصيل البيبلوغرافية
| العنوان: |
Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera. |
| المؤلفون: |
Sun, Hao, Ciska, Malgorzata, Makki, Mongia, Tenllado, Francisco, Canto, Tomás |
| المصدر: |
Molecular Plant Pathology; Jun2024, Vol. 25 Issue 6, p1-15, 15p |
| مصطلحات موضوعية: |
POTATO virus Y, NICOTIANA benthamiana, POXVIRUSES, NON-coding RNA, PLANT viruses, THREONINE, TRANSGENIC plants, AMINO acids |
| مستخلص: |
We had previously reported that a plum pox virus (PPV)‐based chimera that had its P1‐HCPro bi‐cistron replaced by a modified one from potato virus Y (PVY) increased its virulence in some Nicotiana benthamiana plants, after mechanical passages. This correlated with the natural acquisition of amino acid substitutions in several proteins, including in HCPro at either position 352 (Ile→Thr) or 454 (Leu→Arg), or of mutations in non‐coding regions. Thr in position 352 is not found among natural potyviruses, while Arg in 454 is a reversion to the native PVY HCPro amino acid. We show here that both mutations separately contributed to the increased virulence observed in the passaged chimeras that acquired them, and that Thr in position 352 is no intragenic suppressor to a Leu in position 454, because their combined effects were cumulative. We demonstrate that Arg in position 454 improved HCPro autocatalytic cleavage, while Thr in position 352 increased its accumulation and the silencing suppression of a reporter in agropatch assays. We assessed infection by four cloned chimera variants expressing HCPro with none of the two substitutions, one of them or both, in wild‐type versus DCL2/4‐silenced transgenic plants. We found that during infection, the transgenic context of altered small RNAs affected the accumulation of the four HCPro variants differently and hence, also infection virulence. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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