التفاصيل البيبلوغرافية
| العنوان: |
A catalogue of recombination coldspots in interspecific tomato hybrids. |
| المؤلفون: |
Fuentes, Roven Rommel, Nieuwenhuis, Ronald, Chouaref, Jihed, Hesselink, Thamara, van Dooijeweert, Willem, van den Broeck, Hetty C., Schijlen, Elio, Schouten, Henk J., Bai, Yuling, Fransz, Paul, Stam, Maike, de Jong, Hans, Trivino, Sara Diaz, de Ridder, Dick, van Dijk, Aalt D. J., Peters, Sander A. |
| المصدر: |
PLoS Genetics; 7/1/2024, Vol. 20 Issue 7, p1-22, 22p |
| مصطلحات موضوعية: |
TOMATOES, GENETIC variation, TOMATO breeding, GENETIC recombination, CULTIVATED plants, FOOD supply |
| مستخلص: |
Increasing natural resistance and resilience in plants is key for ensuring food security within a changing climate. Breeders improve these traits by crossing cultivars with their wild relatives and introgressing specific alleles through meiotic recombination. However, some genomic regions are devoid of recombination especially in crosses between divergent genomes, limiting the combinations of desirable alleles. Here, we used pooled-pollen sequencing to build a map of recombinant and non-recombinant regions between tomato and five wild relatives commonly used for introgressive tomato breeding. We detected hybrid-specific recombination coldspots that underscore the role of structural variations in modifying recombination patterns and maintaining genetic linkage in interspecific crosses. Crossover regions and coldspots show strong association with specific TE superfamilies exhibiting differentially accessible chromatin between somatic and meiotic cells. About two-thirds of the genome are conserved coldspots, located mostly in the pericentromeres and enriched with retrotransposons. The coldspots also harbor genes associated with agronomic traits and stress resistance, revealing undesired consequences of linkage drag and possible barriers to breeding. We presented examples of linkage drag that can potentially be resolved by pairing tomato with other wild species. Overall, this catalogue will help breeders better understand crossover localization and make informed decisions on generating new tomato varieties. Author summary: Ensuring a stable food supply in a changing climate hinges on enhancing plants' natural defenses and resilience. Breeders achieve this by crossing cultivated plants with their wild counterparts, mixing specific gene variants through genetic recombination. Yet, in some cases, certain genomic areas lack this recombination, particularly when crossing diverse plant types, limiting the mix of beneficial genes. In this study we used a method called pooled-pollen sequencing to map regions where genetic recombination does and doesn't occur when breeding tomatoes with five wild relatives. We discovered specific regions where genetic exchange is rare, mainly due to structural differences in DNA. These regions tend to be rich in repetitive DNA sequences and are less likely to swap genes during reproduction. Importantly, many of these "recombination coldspots" contain genes related to plant health and stress resistance, inadvertently limiting the effectiveness of breeding efforts. By identifying these regions, breeders can now make more informed choices when developing new tomato varieties, potentially by incorporating genes from other wild species to overcome these limitations and improve crop resilience. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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