التفاصيل البيبلوغرافية
| العنوان: |
Altered ontogeny and transcriptomic signatures of tissue-resident pulmonary interstitial macrophages ameliorate allergic airway hyperresponsiveness. |
| المؤلفون: |
Tighe, Robert M., Birukova, Anastasiya, Malakhau, Yuryi, Yoshihiko Kobayashi, Vose, Aaron T., Chandramohan, Vidya, Cyphert-Daly, Jaime M., Cumming, R. Ian, Kirshner, Helene Fradin, Tata, Purushothama R., Ingram, Jennifer L., Gunn, Michael D., Que, Loretta G., Yu, Yen-Rei A. |
| المصدر: |
Frontiers in Immunology; 2024, p1-17, 17p |
| مصطلحات موضوعية: |
HOUSE dust mites, ONTOGENY, ALVEOLAR macrophages, MACROPHAGES, IMMUNOREGULATION |
| مستخلص: |
Introduction: Environmental exposures and experimental manipulations can alter the ontogenetic composition of tissue-resident macrophages. However, the impact of these alterations on subsequent immune responses, particularly in allergic airway diseases, remains poorly understood. This study aims to elucidate the significance of modified macrophage ontogeny resulting from environmental exposures on allergic airway responses to house dust mite (HDM) allergen. Methods: We utilized embryonic lineage labeling to delineate the ontogenetic profile of tissue-resident macrophages at baseline and following the resolution of repeated lipopolysaccharide (LPS)-induced lung injury. We investigated differences in house dust mite (HDM)-induced allergy to assess the influence of macrophage ontogeny on allergic airway responses. Additionally, we employed single-cell RNA sequencing (scRNAseq) and immunofluorescent staining to characterize the pulmonary macrophage composition, associated pathways, and tissue localization. Results: Our findings demonstrate that the ontogeny of homeostatic alveolar and interstitial macrophages is altered after the resolution from repeated LPSinduced lung injury, leading to the replacement of embryonic-derived by bone marrow-derived macrophages. This shift in macrophage ontogeny is associated with reduced HDM-induced allergic airway responses. Through scRNAseq and immunofluorescent staining, we identified a distinct subset of resident-derived interstitial macrophages expressing genes associated with allergic airway diseases, localized adjacent to terminal bronchi, and diminished by prior LPS exposure. Discussion: These results suggest a pivotal role for pulmonary macrophage ontogeny in modulating allergic airway responses. Moreover, our findings highlight the implications of prior environmental exposures in shaping future immune responses and influencing the development of allergies. By elucidating the mechanisms underlying these phenomena, this study provides valuable insights into potential therapeutic targets for allergic airway diseases and avenues for further research into immune modulation and allergic disease prevention. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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