التفاصيل البيبلوغرافية
| العنوان: |
Structures and dynamics of 8-oxo-7,8-dihydro-2′-deoxyguanosine in neutral and basic aqueous solutions by spectroscopy. |
| المؤلفون: |
Wang, Guixiu, Yu, Pengyun, Wang, Jianping |
| المصدر: |
Journal of Chemical Physics; 7/14/2024, Vol. 161 Issue 2, p1-10, 10p |
| مصطلحات موضوعية: |
AQUEOUS solutions, SPECTROMETRY, TAUTOMERISM, NUCLEIC acids, PROTON transfer reactions |
| مستخلص: |
8-oxo-7,8-dihydro-2′-dexyoguanine (8-oxo-dG) can be tautomerized to a 6-enolate,8-keto tautomer through nearby-NH deprotonation at elevated pH. In this work, the N3-protonated 8-oxo-dG tautomers in deuterated pH-buffer solutions were studied using steady-state UV/Vis, FTIR, and ultrafast two-dimensional IR spectroscopies. The presence of 6,8-diketo and C6-anionic tautomers at neutral to basic conditions (pD = 7.4–12.0) was revealed by UV/Vis and FTIR results and was further confirmed by 2D IR signals in both diagonal and off-diagonal regions. However, the C6-enol tautomer, which may be an intermediate during the transition from 6,8-diketo to C6-enolate,C8-keto, was not observed appreciably due to its extreme low population. Furthermore, the neutral-to-anionic tautomeric transition of N3H-8-oxo-dG studied in this work occurs under more basic conditions than the N1H-8-oxo-dG reported previously, showing a higher pKa value for N3H than N1H. Finally, vibrational relaxation of the carbonyl stretching mode was found to be both molecular site dependent and pD dependent for 8oxo-dG. Taken together, this work shows that the ultrafast infrared spectroscopic method is effective for examining tautomers and their dynamics in nucleic acids. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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