التفاصيل البيبلوغرافية
| العنوان: |
Diffusion-tensor magnetic resonance imaging as a non-invasive assessment of extracellular matrix remodeling in lumbar paravertebral muscles of rats with sarcopenia. |
| المؤلفون: |
Huang, Xin-Chen, Ma, Ji-Yao, Gao, Chao, Chen, Jia-Xin, Li, Chun-Li, Huang, Yi-Long, He, Bo |
| المصدر: |
BMC Musculoskeletal Disorders; 7/13/2024, Vol. 25 Issue 1, p1-11, 11p |
| مصطلحات موضوعية: |
MAGNETIC resonance imaging, EXTRACELLULAR matrix, SARCOPENIA, SPRAGUE Dawley rats, PEARSON correlation (Statistics), LUMBAR vertebrae |
| مستخلص: |
Background: Extracellular matrix (ECM) remodeling in skeletal muscle is a significant factor in the development of sarcopenia. This study aims to evaluate changes in ECM remodeling in the lumbar paravertebral muscles of sarcopenic rats using diffusion-tensor magnetic resonance imaging (DT-MRI) and compare them with histology. Methods: Twenty 6-month-old female Sprague Dawley rats were randomly divided into the dexamethasone (DEX) group and the control (CON) group. Both groups underwent 3.0T MRI scanning, including Mensa, T2WI, and DT-MRI sequences. The changes in muscle fibers and extracellular matrix (ECM) of the erector spinal muscle were observed using hematoxylineosin and sirius red staining. The expressions of collagen I, III, and fibronectin in the erector spinae were detected by western blot. Pearson correlation analysis was employed to assess the correlation between MRI quantitative parameters and corresponding histopathology markers. Results: The cross-sectional area and fractional anisotropy values of the erector spinae in the DEX group rats were significantly lower than those in the CON group (p < 0.05). Hematoxylin eosin staining revealed muscle fiber atrophy and disordered arrangement in the DEX group, while sirius red staining showed a significant increase in collagen volume fraction in the DEX group. The western blot results indicate a significant increase in the expression of collagen I, collagen III, and fibronectin in the DEX group (p < 0.001 for all). Correlation coefficients between fractional anisotropy values and collagen volume fraction, collagen I, collagen III, and fibronectin were − 0.71, -0.94, -0.85, and − 0.88, respectively (p < 0.05 for all). Conclusions: The fractional anisotropy value is strongly correlated with the pathological collagen volume fraction, collagen I, collagen III, and fibronectin. This indicates that DT-MRI can non-invasively evaluate the changes in extracellular matrix remodeling in the erector spinal muscle of sarcopenia. It provides a potential imaging biomarker for the diagnosis of sarcopenia. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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