التفاصيل البيبلوغرافية
| العنوان: |
Arabidopsis and maize terminator strength is determined by GC content, polyadenylation motifs and cleavage probability. |
| المؤلفون: |
Gorjifard, Sayeh, Jores, Tobias, Tonnies, Jackson, Mueth, Nicholas A., Bubb, Kerry, Wrightsman, Travis, Buckler, Edward S., Fields, Stanley, Cuperus, Josh T., Queitsch, Christine |
| المصدر: |
Nature Communications; 7/12/2024, Vol. 15 Issue 1, p1-21, 21p |
| مصطلحات موضوعية: |
ARABIDOPSIS, ARABIDOPSIS thaliana, GENETIC translation, PROBABILITY theory, BIOLOGY, COMPUTATIONAL neuroscience |
| مستخلص: |
The 3' end of a gene, often called a terminator, modulates mRNA stability, localization, translation, and polyadenylation. Here, we adapted Plant STARR-seq, a massively parallel reporter assay, to measure the activity of over 50,000 terminators from the plants Arabidopsis thaliana and Zea mays. We characterize thousands of plant terminators, including many that outperform bacterial terminators commonly used in plants. Terminator activity is species-specific, differing in tobacco leaf and maize protoplast assays. While recapitulating known biology, our results reveal the relative contributions of polyadenylation motifs to terminator strength. We built a computational model to predict terminator strength and used it to conduct in silico evolution that generated optimized synthetic terminators. Additionally, we discover alternative polyadenylation sites across tens of thousands of terminators; however, the strongest terminators tend to have a dominant cleavage site. Our results establish features of plant terminator function and identify strong naturally occurring and synthetic terminators. Using Plant STARR-seq, Gorjifard et al analyzed over 50,000 plant terminators and found that plant terminator activity is species-specific, highly dependent on GC content and polyadenylation motifs, and they developed a computational model to predict terminator strength and create optimized synthetic terminators. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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