التفاصيل البيبلوغرافية
| العنوان: |
Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii. |
| المؤلفون: |
Severinsen, Manja Mølgaard, Bachleitner, Simone, Modenese, Viola, Ata, Özge, Mattanovich, Diethard |
| المصدر: |
Biotechnology for Biofuels & Bioproducts; 7/15/2024, Vol. 17 Issue 1, p1-14, 14p |
| مصطلحات موضوعية: |
ITACONIC acid, BIOCHEMICAL substrates, SUSTAINABILITY, ASPERGILLUS terreus, ENERGY consumption, METHANOL |
| مستخلص: |
Background: Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single-carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially, methanol is gaining prominence for bio-production of commodity chemicals. Results: In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g·L−1 after 5 days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies, we further boosted the itaconic acid production reaching titers of 55 g·L−1 after less than 5 days of methanol feed, while increasing the product yield on methanol from 0.06 g·g−1 to 0.24 g·g−1. Conclusion: Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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