التفاصيل البيبلوغرافية
| العنوان: |
First report on the presence of potentially pathogenic Vibrio parahaemolyticus in farmed mussels (Mytilus galloprovincialis) from the Istrian aquatorium by microbiological and molecular methods. |
| Alternate Title: |
Prvo izvješće o prisutnosti potencijalno patogene bakterije Vibrio parahaemolyticus u uzgojenih dagnji (Mytilus galloprovincialis) istarskoga akvatorija primjenom mikrobioloških i molekularnih metoda. (Croatian) |
| المؤلفون: |
Džafić, N., Begić, B. Boljkovac, Barać, K. N., Benić, M., Duvnjak, S., Kozačinski, L., Kvrgić, K., Humski, A. |
| المصدر: |
Veterinarska Stanica; 2025, Vol. 56 Issue 1, p1-12, 12p |
| مصطلحات موضوعية: |
VIBRIO parahaemolyticus, MYTILUS galloprovincialis, POLYMERASE chain reaction, MOLECULAR diagnosis, HEMOLYSIS & hemolysins |
| Abstract (English): |
Microbiological and molecular PCR techniques were used to analyse 328 samples of mussels (Mytilus galloprovincialis) cultured in Istrian waters. Molecular testing was more effective than microbiological testing, detecting the presence of the bacterium Vibrio parahaemolyticus in 21.34% of samples, compared to 2.44% detected through microbiological methods. The probability of detecting the presence of this bacterium was 7.19 times higher when the sea surface temperature was above 15°C, while thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) positive samples were detected at sea temperatures above 22.5°C and 24.7°C, respectively. TDH- and TRH-positive samples constituted 10% of the total V. parahaemolyticus-positive samples. As there is no legal obligation to monitor the presence of this bacterium, consuming raw or insufficiently cooked mussels can lead to illness. [ABSTRACT FROM AUTHOR] |
| Abstract (Croatian): |
Primjenom mikrobioloških i molekularnih tehnika, pretraženo je 328 uzoraka školjkaša dagnji (Mytilus galloprovincialis), uzgojenih na području istarskoga akvatorija. Rezultati ukazuju na smanjenu osjetljivost mikrobiološke tehnike (2.44 %) u odnosu na molekularnu dijagnostiku kojom je dokazana prisutnost bakterije V. parahaemolyticus u 21,34 % uzoraka. Izgledi za dokazivanje prisutnosti ove bakterije su 7,19 puta veći kada je površinska temperatura mora veća od 15 °C, dok su geni za kodiranje termostabilnog direktnog hemolizina (TDH) i hemolizina ovisnog o TDH (TRH) u uzorcima dokazani pri temperaturama mora iznad 22,5 °C, odnosno 24,7 °C. Udio TDH i TRH pozitivnih uzoraka iznosio je 10 % od ukupnih 70 V. parahaemolyticus-pozitivnih uzoraka. S obzirom na nedostatak zakonske obveze praćenja prisutnosti ove bakterije moguće je oboljevanje ljudi nakon konzumacije sirovih ili termički nedostatno obrađenih školjkaša. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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