التفاصيل البيبلوغرافية
| العنوان: |
Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals. |
| المؤلفون: |
Cai, S. H., Chen, Z., Sun, L. J., Zhong, J., Huang, Y. C., Yang, S. P., Lu, Y. S. |
| المصدر: |
Applied Biochemistry & Microbiology; Aug2024, Vol. 60 Issue 4, p749-756, 8p |
| مصطلحات موضوعية: |
VIBRIO harveyi, AQUATIC animals, WHITELEG shrimp, BETAINE, GROUPERS, VIBRIO |
| مستخلص: |
Vibrio harveyi is a normal flora present in seawater, but in recent years, with large density aquaculture, V. harveyi has become one of the causative organisms in fish and shrimp. In this study, V. harveyiVieA gene was used as the target to design primers and establish the loop-mediated isothermal amplification (LAMP) combined with visual lateral flow test strip (LFD). The optimal reaction conditions were optimized as 60°C, 45 min, 1.2 mM Mg2+, 0.64 mM dNTPs, 0.25 mM betaine, and 16:1 ratio of internal/external primers. The results showed that the established LAMP assay was able to specifically detect V. harveyi with a sensitivity of 3.4 × 10–5 ng/µL. Clinical applicability analysis revealed that only V. harveyi was detected in the samples for pearl gentian grouper and whiteleg shrimp artificially infected by Vibrio. Therefore, we established a visual, reliable, rapid and sensitive LAMP-LFD method for detecting V. harveyi in aquatic animals. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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