التفاصيل البيبلوغرافية
| العنوان: |
Mutations of Single Residues in the Complexin N-terminus Exhibit Distinct Phenotypes in Synaptic Vesicle Fusion. |
| المؤلفون: |
Toulme, Estelle, Murach, Jacqueline, Bärfuss, Simon, Kroll, Jana, Malsam, Jörg, Trimbuch, Thorsten, Herman, Melissa A., Söllner, Thomas H., Rosenmund, Christian |
| المصدر: |
Journal of Neuroscience; 7/31/2024, Vol. 44 Issue 31, p1-16, 16p |
| مصطلحات موضوعية: |
SYNAPTIC vesicles, PHENOTYPES, PROTEIN receptors, AMINO acids, MEMBRANE fusion, CALCIUM channels, NEURAL transmission, SYNAPSES |
| مستخلص: |
The release of neurotransmitters (NTs) at central synapses is dependent on a cascade of protein interactions, specific to the presynaptic compartment. Among those dedicated molecules, the cytosolic complexins play an incompletely defined role as synaptic transmission regulators. Complexins are multidomain proteins that bind soluble N-ethylmaleimide sensitive factor attachment protein receptor complexes, conferring both inhibitory and stimulatory functions. Using systematic mutagenesis and comparing reconstituted in vitro membrane fusion assays with electrophysiology in cultured neurons from mice of either sex, we deciphered the function of the N-terminus of complexin (Cpx) II. The N-terminus (amino acid 1-27) starts with a region enriched in hydrophobic amino acids (1-12), which binds lipids. Mutants maintaining this hydrophobic character retained the stimulatory function of Cpx, whereas exchanges introducing charged residues perturbed both spontaneous and evoked exocytosis. Mutants in the more distal region of the N-terminal domain (amino acid 11-18) showed a spectrum of effects. On the one hand, mutation of residue A12 increased spontaneous release without affecting evoked release. On the other hand, replacing D15 with amino acids of different shapes or hydrophobic properties (but not charge) not only increased spontaneous release but also impaired evoked release. Most surprising, this substitution reduced the size of the readily releasable pool, a novel function for Cpx at mammalian synapses. Thus, the exact amino acid composition of the Cpx N-terminus fine-tunes the degree of spontaneous and evoked NT release. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
