التفاصيل البيبلوغرافية
| العنوان: |
Cytotoxicity Screening of Endophytic Fungi from Phaleria macrocarpa (Scheff) Boerl) Collected in Yogyakarta District. |
| المؤلفون: |
Agustina, Ikra, Lay, Caterina Siskadewi, Astuti, Puji, Hertiani, Triana |
| المصدر: |
Indonesian Journal of Pharmacy / Majalah Farmasi Indonesia; Apr-Jun2024, Vol. 35 Issue 2, p219-226, 8p |
| مصطلحات موضوعية: |
ENDOPHYTIC fungi, CYTOTOXINS, METABOLITES, CELL cycle, DISEASE prevalence |
| مستخلص: |
Cancer is a disease with a high prevalence and mortality rate globally. Indonesia's biodiversity has potential to be a source of medicinal ingredients. Phaleria macrocarpa (Scheff) Boerl is known to contain active metabolites with potential anticancer properties. Some endophytes are reported to be able to produce secondary metabolites similar to those of their parent plants. This study aimed to obtain endophytic fungi from P. macrocarpa that can produce compounds with anticancer properties. Endophytes producing such compounds were explored by screening endophytic fungi producing secondary metabolites from parts P. macrocarpa that was collected from the Yogyakarta area. The anticancer bioassay was performed in vitro using cancer cells T47D, MCF7, HeLa, and normal Vero cells. Potential extracts were traced for their mechanisms of cell cycle modulation and apoptosis using flow cytometry. Fungal identification was conducted on fungi producing potential cytotoxic extracts. The compound content was then analyzed using LC-HRMS (Liquid Chromatography-High Resolution Mass Spectrometry). Results from the six extracts obtained from fruit, leaf, and stem parts showed that the endophytic extract from the leaves (code PC-L2) had the best cytotoxicity compared to other extracts and demonstrated the best result on MCF-7 cells with an IC50 value of 110.66 µg/mL. The produced PC-L2 extract fungi are closely associated with Clonostachys wenpingii strain DUCC5606. This extract induced apoptosis, but not through the mechanism of cell cycle modulation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
