التفاصيل البيبلوغرافية
| العنوان: |
Preliminary study on the mechanism of PANoptosis occurrence in testicular tissue of type I diabetes mellitus. (English) |
| المؤلفون: |
Du Zijun, Chen Ying, Huang Zhansen, Zhang Zebo, Wang Wei, Tang Bowen, Li Hao, Feng Jiaxin, Su Yiwen, Yuan Sushun, Chen Diling, Yuan Qilong, Chen Jun |
| المصدر: |
Chinese Journal of Andrology; 2024, Vol. 38 Issue 4, p15-23, 9p |
| مصطلحات موضوعية: |
TYPE 1 diabetes, ANIMAL experimentation, GENE expression |
| مستخلص: |
Objective To explore the possible mechanism of PANoptosis ocurrence in testicular tissue of type I diabetes mellitus (T1DM). Methods Fifty SPF-grade SD rats were randomly divided into the control group (n m 15) and type 1 diabetic group (n m 35). After 8 weeks of successful modeling, testicular tissues were collected for detecting PANoptosis- related indexes as well as PI3K, AKT and NF-κB expressions. GC-1 spg cells were divided into a control group, a hyperglycemic group (HG), and a LY294002 and hyperglycemic group (LY294002 a HG) ; CCK-8 experiments were used to determine the optimal concentration and time of PI3K inhitbitor (LY294002) ; Intracellular PANoptosis-related indexes and PI3K, AKT, NF-κB expressions were examined, respectively. Results For in vivo animal experiments, PANoptosis-related immunohistochemical indexes, mRNA and protein expressions in testicular tissues in the T1DM group were all significantly higher than those in control group; Immunohistochemical indexes, mRNA and protein expressions related to PI3KAKT/NF-κB signaling pathway were also elevated in testicular tissues in the T1DM group. For in vitro cell tests, caspase-1 expression within the GC-1 spg cells was upregulated in the HG group compared with the control group; The expressions of PANoptosis-related proteins and PI3K, AKT and NF-κB were increased in GC-1 spg cells of the HG group; But the expressions of the above proteins were al decreased in the LY294002 a HG group compared with the HG group. Conclusion The occurrence of PANoptosis in testicular tissue of type I diabetic rats may be mediated by the activation of PI3KAKT/NF-κB signaling pathway. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
