التفاصيل البيبلوغرافية
| العنوان: |
PLGA-LEC/F127 hybrid nanoparticles loaded with curcumin and their modulatory effect on monocytes. |
| المؤلفون: |
T Cruz, Jennifer, Karen Álvarez, H Orozco, Víctor, Mauricio Rojas, A Morales-Luckie, Raul, F Giraldo, Luis |
| المصدر: |
Nanomedicine; 2024, Vol. 19 Issue 15, p1407-1423, 17p |
| مستخلص: |
Aim: To investigate the effect of surfactant type on curcumin-loaded (CUR) PLGA nanoparticles (NPs) to modulate monocyte functions. Materials & methods: The nanoprecipitation method was used, and PLGA NPs were designed using Pluronic F127 (F127) and/or lecithin (LEC) as surfactants. Results: The Z-average of the NPs was <200 nm, they had a spherical shape, Derjaguin–Muller–Toporov modulus >0.128 MPa, they were stable during storage at 4°C, ζ-potential ∼-40 mV, polydispersity index <0.26 and % EE of CUR >94%. PLGA-LEC/F127 NPs showed favorable physicochemical and nanomechanical properties. These NPs were bound and internalized mainly by monocytes, suppressed monocyte-induced reactive oxygen species production, and decreased the ability of monocytes to modulate T-cell proliferation. Conclusion: These results demonstrate the potential of these NPs for targeted therapy. This study explores how different surfactants affect curcumin-loaded PLGA nanoparticles, a biodegradable polymer. The nanoparticles were designed using Pluronic F127 and/or lecithin as surfactants. They are less than 200 nm and spherical. They are stable when stored at 4 °C, with a surface charge of about -40 mV, and can encapsulate more than 94% of curcumin. The results of this study are promising, showing that PLGA nanoparticles using a mixture of lecithin and Pluronic F127 as surfactants have favorable properties toward monocyte adhesion. They are primarily taken up by monocytes, a type of white blood cell, and demonstrate a remarkable ability to reduce the production of reactive oxygen species, which can cause cell damage, as well as the ability of monocytes to stimulate the proliferation of T cells. This underscores the potential of these nanoparticles in targeted therapy, particularly in diseases where monocytes play a pivotal role, such as chronic inflammatory conditions. GRAPHICAL ABSTRACT Article highlights The consistency in size and ζ-potential across different nanoparticle formulations underscores the LEC/F127 mixture (70:30 wt/wt) potential in producing hybrid platforms with optimal physicochemical properties for biomedical applications. PLGA-LEC/F127 nanoparticles (NPs) showed higher hardness (Derjaguin–Muller–Toporov [DMT] = 0.406 MPa) compared with naked ones (DMT = 0.128 MPa) (Table 2), indicating that surfactant presence alters NPs elastic properties, increasing their hardness and cellular internalization. PLGA-LEC and PLGA-LEC/F127 NPs were preferentially bound/internalized by monocytes compared with neutrophils and lymphocytes. F127/LEC NPs did not significantly affect the mitochondrial membrane potential or plasma membrane integrity of peripheral blood mononuclear cells. Curcumin (CUR) was efficiently encapsulated within F127/LEC NPs. Free and encapsulated CUR reduced monocytes' ability to support autologous T-cell proliferation. Encapsulated CUR inhibited reactive oxygen species production by phorbol myristate acetate-stimulated monocytes. In some cases, encapsulating CUR in PLGA-LEC/F127 NPs enhances its inhibitory effects. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nanomedicine is the property of Future Medicine Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
PDF full text from Publisher