التفاصيل البيبلوغرافية
| العنوان: |
Early Detection of Food Safety and Spoilage Incidents Based on Live Microbiome Profiling and PMA-qPCR Monitoring of Indicators. |
| المؤلفون: |
Cohen Hakmon, May, Buhnik-Rosenblau, Keren, Hanani, Hila, Korach-Rechtman, Hila, Mor, Dagan, Etkin, Erez, Kashi, Yechezkel |
| المصدر: |
Foods; Aug2024, Vol. 13 Issue 15, p2459, 16p |
| مصطلحات موضوعية: |
PROPIDIUM monoazide, FOOD spoilage, DETECTION of microorganisms, FOOD pathogens, FOOD production |
| مستخلص: |
The early detection of spoilage microorganisms and food pathogens is of paramount importance in food production systems. We propose a novel strategy for the early detection of food production defects, harnessing the product microbiome. We hypothesize that by establishing microbiome datasets of proper and defective batches, indicator bacteria signaling production errors can be identified and targeted for rapid quantification as part of routine practice. Using the production process of pastrami as a model, we characterized its live microbiome profiles throughout the production stages and in the final product, using propidium monoazide treatment followed by 16S rDNA sequencing. Pastrami demonstrated product-specific and consistent microbiome profiles predominated by Serratia and Vibrionimonas, with distinct microbial signatures across the production stages. Based on the established microbiome dataset, we were able to detect shifts in the microbiome profile of a defective batch produced under lactate deficiency. The most substantial changes were observed as increased relative abundances of Vibrio and Lactobacillus, which were subsequently defined as potential lactate-deficiency indicators. PMA-qPCR efficiently detected increased levels of these species, thus proving useful in rapidly pinpointing the production defect. This approach offers the possibility of the in-house detection of defective production events with same-day results, promoting safer food production systems. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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