التفاصيل البيبلوغرافية
| العنوان: |
AlphaFold2-guided engineering of split-GFP technology enables labeling of endogenous tubulins across species while preserving function. |
| المؤلفون: |
Xu, Kaiming, Li, Zhiyuan, Mao, Linfan, Guo, Zhengyang, Chen, Zhe, Chai, Yongping, Xie, Chao, Yang, Xuerui, Na, Jie, Li, Wei, Ou, Guangshuo |
| المصدر: |
PLoS Biology; 8/19/2024, Vol. 22 Issue 8, p1-27, 27p |
| مصطلحات موضوعية: |
CELL physiology, MISSENSE mutation, CAENORHABDITIS elegans, MICROTUBULES, EMBRYOLOGY, TUBULINS |
| مستخلص: |
Dynamic properties are essential for microtubule (MT) physiology. Current techniques for in vivo imaging of MTs present intrinsic limitations in elucidating the isotype-specific nuances of tubulins, which contribute to their versatile functions. Harnessing the power of the AlphaFold2 pipeline, we engineered a strategy for the minimally invasive fluorescence labeling of endogenous tubulin isotypes or those harboring missense mutations. We demonstrated that a specifically designed 16-amino acid linker, coupled with sfGFP11 from the split-sfGFP system and integration into the H1-S2 loop of tubulin, facilitated tubulin labeling without compromising MT dynamics, embryonic development, or ciliogenesis in Caenorhabditis elegans. Extending this technique to human cells and murine oocytes, we visualized MTs with the minimal background fluorescence and a pathogenic tubulin isoform with fidelity. The utility of our approach across biological contexts and species set an additional paradigm for studying tubulin dynamics and functional specificity, with implications for understanding tubulin-related diseases known as tubulinopathies. GFP-tagged tubulins have been widely used to monitor and visualize tubulin isotypes, but this is known to interfere with their cellular functions. This study describes a functional labeling strategy, guided by AlphaFold2 structural predictions, to label endogenous tubulins across diverse species while preserving functionality. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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