التفاصيل البيبلوغرافية
| العنوان: |
In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window. |
| المؤلفون: |
Jiang, Longjie, Yang, Jie, Gao, Xiujuan, Huang, Jiangfeng, Liu, Qian, Fu, Ling |
| المصدر: |
Nature Communications; 9/16/2024, Vol. 15 Issue 1, p1-15, 15p |
| مصطلحات موضوعية: |
ENTERIC nervous system, GASTROINTESTINAL system, GASTROINTESTINAL motility, VAGUS nerve, ABDOMINAL wall |
| مستخلص: |
The vagus nerve (VN) extensively innervates the gastric enteric nervous system (ENS), but its influence on gastric ENS functionality and motility in vivo remains unclear due to technical challenges. Here we describe a method for stable, long-term observation of gastric ENS activity and muscle dynamics at cellular resolution, which can also be extended to intestinal applications. This method involves ENS-specific labeling and the implantation of an abdominal wall window for optical recording in male mice. In vivo calcium imaging reveals a linear relationship between vagal stimulation frequency and myenteric neuron activation in gastric antrum. Furthermore, the motility of gastric antrum is significantly enhanced and shows a positive correlation with the intensity and number of activated myenteric neurons. While vagal stimulation also activates proximal colonic myenteric neurons, this activation is not frequency-dependent and does not induce proximal colonic motility. The method and results provide important insights into VN-ENS interactions in vivo, advancing our understanding of gastrointestinal motility regulation. In vivo optical recording of the real-time effects of extrinsic afferents on the activity of the enteric nervous system has been challenging. Here, the authors show a method that allows long-term imaging of the mouse gastric antrum and stable recording of the gastric myenteric plexus in response to vagal activation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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