التفاصيل البيبلوغرافية
| العنوان: |
Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata. |
| المؤلفون: |
Judice, C. C., Carazzole, M. F., Festa, F., Sogayar, M. C., Hartfelder, K., Pereira, G. A. G. |
| المصدر: |
Insect Molecular Biology; Feb2006, Vol. 15 Issue 1, p33-44, 12p, 4 Graphs |
| مصطلحات موضوعية: |
GENE expression, MELIPONA, STINGLESS bees, HONEYBEES, INSECT populations, MOLECULAR biology |
| مستخلص: |
To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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