التفاصيل البيبلوغرافية
| العنوان: |
An efficient rapid system for profiling the cellular activities of molecular libraries. |
| المؤلفون: |
Melnick, Jonathan S., Janes, Jeff, Sungjoon Kim, Chang, Jim Y., Sipes, Daniel G., Gunderson, Drew, James, Laura, Matzen, Jason T., Garcia, Michael E., Hood, Tami L., Ronak Beigi, Gang Xia, Harig, Richard A., Asatryan, Hayk, Yan, S. Frank, Yingyao Zhou, Xiang-Ju Gu, Saadat, Alham, Zhou, Vicki, King, Frederick J. |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 2/28/2006, Vol. 103 Issue 9, p3153-3158, 6p, 1 Color Photograph, 2 Diagrams, 1 Graph |
| مصطلحات موضوعية: |
CYTOLOGY, MOLECULAR pharmacology, DRUG development, MOLECULES, PEPTIDES, RNA, FIRE assay, PROTEIN-tyrosine kinases, QUANTITATIVE research |
| مستخلص: |
Rapid quantitative methods for characterizing small molecules, peptides, proteins, or RNAs in a broad array of cellular assays would allow one to discover new biological activities associated with these molecules and also provide a more comprehensive profile of drug candidates early in the drug development process. Here we describe a robotic system, termed the automated compound profiler, capable of both propagating a large number of cell lines in parallel and assaying large collections of molecules simultaneously against a matrix of cellular assays in a highly reproductible manner. To illustrate its utility, we have characterized a set of 1,400 kinase inhibitors in a panel of 35 activated tyrosine-kinase-dependent cellular assays in dose-response format in a single experiment. Analysis of the resulting multidimensional dataset revealed subclusters of both inhibitors and kinases with closely correlated activities. The approach also identified activities for the p38 inhibitor B1RB796 and the dual src/abl inhibitor BMS-354825 and exposed the expected side activities for Glivec/ST1571, including cellular inhibition of c-kit and platelet-derived growth factor receptor. This methodology provides a powerful tool for unraveling the cellular biology and molecular pharmacology of both naturally occurring and synthetic chemical diversity. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
