التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of zinc salt based fixatives for preserving antigenic determinants for immunohistochemical demonstration of murine immune system cell markers. |
| المؤلفون: |
Hicks, Dj, Johnson, L, Mitchell, Sm, Gough, J, Cooley, Wa, La Ragione, Rm, Spencer, Yi, Wangoo, A |
| المصدر: |
Biotechnic & Histochemistry; Jan/Feb2006, Vol. 81 Issue 1, p23-30, 8p |
| مصطلحات موضوعية: |
EPITOPES, IMMUNOHISTOCHEMISTRY, IMMUNE system, ZINC salts, MICE |
| مستخلص: |
Conventional aldehyde based fixatives produce good morphological preservation. However, owing to their cross-linking mechanism of action, epitope loss may occur during fixation compromising the tissue for subsequent immunohistochemical (IHC) analysis. IHC is an important tool for characterizing antigen, cytokine and cytomorphological markers. The increasing use of mouse models for study of pathogenesis has highlighted the need to investigate alternative fixatives. In the study reported here, tissue samples from RIII mice with immune mediated lesions, Mycobacterium bovis infected mice, and uninfected control mice were fixed in either zinc salt fixative or buffered formalin, then tested for IHC using a panel of antibodies (CD3, CD4, CD8, CD45, CD54, F4/80, Interferon-gamma and MIP2). Zinc salt fixation preserved processing-sensitive murine cell markers (CD4, CD8 and CD54) and improved the intensity of immunolabeling for CD45, F4/80 and CD3. Buffered formalin failed to preserve any of the processing-sensitive murine epitopes for demonstration by subsequent IHC. [ABSTRACT FROM AUTHOR] |
|
Copyright of Biotechnic & Histochemistry is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
PDF full text from Publisher 
Full Text Finder