التفاصيل البيبلوغرافية
| العنوان: |
Effects of Chlorine Injury, Starvation, and ColitagTM Enrichment on Sandwich Enzyme-Linked Immunosorbent Assay (sELISA) Detection of Escherichia coli O157:H7. |
| المؤلفون: |
Nyquist‐Battie, Cynthia, Freeman, Laura, Soberano, John‐Paul, Lund, Deanna, Kearns, Elizabeth, Lim, DanielV. |
| المصدر: |
Journal of Immunoassay & Immunochemistry; Nov2007, Vol. 28 Issue 4, p371-383, 13p, 2 Charts, 3 Graphs |
| مصطلحات موضوعية: |
ENZYME-linked immunosorbent assay, ESCHERICHIA coli, MATRICES (Mathematics), FOODBORNE diseases, THIOSULFATES |
| مستخلص: |
The validity of ELISA for detection of E. coli O157:H7 under many conditions is not proven. In this work, sELISA was able to detect bacteria after sub-lethal chlorine exposure and after seven days of starvation with little to no change in limit of detection and fluorescence signal as long as chlorine was not present in the sample or was neutralized by sodium thiosulfate. After Colitag™ enrichment, sELISA detected ∼3 colony forming units/ml of originally added E. coli O157:H7. Thus, the present sELISA is valid for detection of E. coli O157:H7 in water sources, although sample matrices may interfere with assay. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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