التفاصيل البيبلوغرافية
| العنوان: |
Dynamics of insulin signalling in liver during hyperinsulinemic euglycaemic clamp conditions in vivo and the effects of high-fat feeding in male mice. |
| المؤلفون: |
Korsheninnikova, E., Voshol, P. J., Baan, B., van der Zon, G. C. M., Havekes, L. M., Romijn, J. A., Maassen, J. A., Ouwens, D. M. |
| المصدر: |
Archives of Physiology & Biochemistry; Oct2007, Vol. 113 Issue 4/5, p173-185, 13p, 1 Black and White Photograph, 4 Charts, 6 Graphs |
| مصطلحات موضوعية: |
INSULIN, CARBOHYDRATES, LIPIDS, PROTEIN metabolism, INSULIN resistance, PHOSPHORYLATION |
| مستخلص: |
Insulin is an important regulator of hepatic carbohydrate, lipid, and protein metabolism, and the regulation of these processes by insulin is disturbed under conditions of insulin resistance and type 2 diabetes. Despite these alterations, the impact of insulin resistance on insulin signalling in the liver is not well defined. Variations in time and dose of insulin stimulation as well as plasma glucose levels may underlie this. The present study aimed at determining the dynamics of activation of hepatic insulin signalling in vivo at insulin concentrations resembling those achieved after a meal, and addressing the effects of high-fat feeding. An unexpected finding of this study was the biphasic activation pattern of the IRS-PI3K-PKB/Akt pathway. Our findings indicate that the first burst of activation contributes to regulation of glucose metabolism. The physiological function of the second peak is still unknown, but may involve regulation of protein synthesis. Finally, high-fat feeding caused hepatic insulin resistance, as illustrated by a reduced suppression of hepatic glucose production. A sustained increased phosphorylation of the serine/threonine kinases p70S6kinase and Jun N-terminal kinase in the absence of insulin may underlie the abrogated phosphorylation of the IRS proteins and their downstream targets. [ABSTRACT FROM AUTHOR] |
|
Copyright of Archives of Physiology & Biochemistry is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
PDF full text from Publisher