التفاصيل البيبلوغرافية
| العنوان: |
Development and Validation of a Fast RP-HPLC Method for Determination of Methotrexate Entrapment Efficiency in Polymeric Nanocapsules. |
| المؤلفون: |
Tatiane Sartori, Fabio Seigi Murakami, Ariane Pinheiro Cruz, Angela Machado de Campos |
| المصدر: |
Journal of Chromatographic Science; Jul2008, Vol. 46 Issue 6, p505-509, 5p |
| مصطلحات موضوعية: |
ANTIMETABOLITES, HIGH performance liquid chromatography, ACETONITRILE, TETRAHYDROFURAN, POLYMERS, ANTIMITOTIC agents |
| مستخلص: |
A rapid and effective isocratic chromatographic procedure is successfully developed to determinate methotrexate (MTX) entrapment efficiency (EE) in polymeric nanocapsules using reversed-phase high-performance liquid chromatography. The method employed a RP-C18 Shimadzu Shim-pack CLC-ODS (150 mm × 4.6 mm, 5 µm) column with mobile phase constituted by a mixture of water–acetonitrile–tetrahydrofuran (65:30:5 v/v/v; pH 3.0) at a flow rate of 0.8 mL/min. The eluate is monitored with a UV detector set at 313 nm. The parameters used in the validation process are: linearity, specificity, precision, accuracy, and limit of quantitation (LOQ). The linearity is evaluated by a calibration curve in the concentration range of 10–50 µg/mL and presented a correlation coefficient of 0.9998. The polymers (PLA or PLA-PEG), oil, and surfactants used in the nanocapsule formulation did not interfere with analysis and the recovery was quantitative. The intra and inter-day assay relative standard deviation were less than 0.72%. Results are satisfactory, and the method proved to be adequate for the determination of methotrexate in nanocpsules formulations. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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