التفاصيل البيبلوغرافية
| العنوان: |
Effect of HO-1 cDNA-liposome complex transfer on erectile signalling of aged rats. |
| المؤلفون: |
Abdel Aziz, M. T., Mostafa, T., Atta, H., Mahfouz, S., Wassef, M., Fouad, H., Kamel, M., Rashed, L., Sabry, D., Mouhamed, O. |
| المصدر: |
Andrologia; Jun2009, Vol. 41 Issue 3, p176-183, 8p, 3 Black and White Photographs, 4 Graphs |
| مصطلحات موضوعية: |
LABORATORY rats, THERAPEUTIC use of carbon monoxide, IMPOTENCE, HEME oxygenase, WESTERN immunoblotting, PENIS physiology, GENE therapy, PHYSIOLOGY, THERAPEUTICS |
| مستخلص: |
This work aimed to assess the efficacy of haeme oxygenase-1 (HO-1) cDNA-liposome complex transfer as a mediator of erectile signalling in aged rats. One hundred and fifty aged white albino rats were equally divided into five groups: controls, rats receiving lipofectamine, rats receiving intracorporeal HO-1 cDNA-lipsome complex, rats receiving HO-1 cDNA-liposome complex plus nitric oxide synthase (NOS) inhibitor, and rats receiving HO-1 cDNA-liposome complex plus HO inhibitor. Six rats were killed from each group after 12, 24 and 48 h, and after1 and 2 weeks. In dissected cavernous tissues, the following were assessed: HO-1 gene expression, Western blot for HO-1, HO enzyme activity, cGMP and histopathology. The results showed that HO-1 cDNA-liposome complex transfer led to a significant increase in cavernous tissue HO-1 protein, HO-1 gene expression, HO enzyme activity and cGMP up to 1 week. NOS inhibition exhibited no effect on HO-1 gene enhancement of cavernous tissue HO enzyme activity or cGMP, whereas inhibition of HO significantly decreased these parameters. Histopathology of cavernous tissue demonstrated a significant dilatation of helicine arteries in HO-1 cDNA-liposome complex treated group after 48 h compared with the controls. It is concluded that HO-1 cDNA-liposome complex transfer augments cavernous tissue cGMP with subsequent sinusoidal relaxation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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