التفاصيل البيبلوغرافية
العنوان: |
Biochemical and Functional Characterization of BLUF-Type Flavin-Binding Proteins of Two Species of Cyanobacteria. |
المؤلفون: |
Okajima, Koji, Yoshihara, Shizue, Fukushima, Yoshimasa, Xiaoxing Geng, Katayama, Mitsunori, Higashi, Shoichi, Watanabe, Masakatsu, Sato, Shusei, Tabata, Satoshi, Shibata, Yutaka, Itoh, Shigeru, Ikeuchi, Masahiko |
المصدر: |
Journal of Biochemistry; Jun2005, Vol. 137 Issue 6, p741-750, 10p |
مصطلحات موضوعية: |
CYANOBACTERIA, PHOTOTROPISM, BIOMOLECULES, PHOTOBIOLOGY, PROKARYOTES |
مستخلص: |
BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, Tll0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared Tll0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. Tll0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the “short” BLUF proteins in the regulation of cyanobacterial phototaxis. [ABSTRACT FROM PUBLISHER] |
|
Copyright of Journal of Biochemistry is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
قاعدة البيانات: |
Complementary Index |