التفاصيل البيبلوغرافية
| العنوان: |
Biochemical and Functional Characterization of BLUF-Type Flavin-Binding Proteins of Two Species of Cyanobacteria. |
| المؤلفون: |
Okajima, Koji, Yoshihara, Shizue, Fukushima, Yoshimasa, Xiaoxing Geng, Katayama, Mitsunori, Higashi, Shoichi, Watanabe, Masakatsu, Sato, Shusei, Tabata, Satoshi, Shibata, Yutaka, Itoh, Shigeru, Ikeuchi, Masahiko |
| المصدر: |
Journal of Biochemistry; Jun2005, Vol. 137 Issue 6, p741-750, 10p |
| مصطلحات موضوعية: |
CYANOBACTERIA, PHOTOTROPISM, BIOMOLECULES, PHOTOBIOLOGY, PROKARYOTES |
| مستخلص: |
BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, Tll0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared Tll0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. Tll0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the “short” BLUF proteins in the regulation of cyanobacterial phototaxis. [ABSTRACT FROM PUBLISHER] |
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| قاعدة البيانات: |
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