التفاصيل البيبلوغرافية
| العنوان: |
The BTB/POZ Domain of the Arabidopsis Disease Resistance Protein NPR1 Interacts with the Repression Domain of TGA2 to Negate Its Function. |
| المؤلفون: |
Boyle, Patrick, Le Su, Errol, Rochon, Amanda, Shearer, Heather L., Murmu, Jhadeswar, Fobert, Jee Pierre R., Després, Charles, Jee Yan Chu |
| المصدر: |
Plant Cell; Nov2009, Vol. 21 Issue 11, p3700-3713, 14p, 1 Diagram, 6 Graphs |
| مصطلحات موضوعية: |
ARABIDOPSIS, DISEASE resistance of plants, PLANT proteins, ARABIDOPSIS thaliana, DNA, DIMERS, OLIGOMERS |
| مستخلص: |
TGA2 and NONEXPRESSER OF PR GENES1 (NPR1) are activators of systemic acquired resistance (SAR) and of the SAR marker gene pathogenesis-related-1 (PR-1) in Arabidopsis thaliana. TGA2 is a transcriptional repressor required for basal repression of PR-1, but during SAR, TGA2 recruits NPR1 as part of an enhanceosome. Transactivation by the enhanceosome requires the NPR1 BTB/POZ domain. However, the NPR1 BTB/POZ domain does not contain an autonomous transactivation domain; thus, its molecular role within the enhanceosome remains elusive. We now show by gel filtration analyses that TGA2 binds DNA as a dimer, tetramer, or oligomer. Using in vivo plant transcription assays, we localize the repression domain of TGA2 to the N terminus and demonstrate that this domain is responsible for modulating the DNA binding activity of the oligomer both in vitro and in vivo. We confirm that the NPR1 BTB/POZ domain interacts with and negates the molecular function of the TGA2 repression domain by excluding TGA2 oligomers from cognate DNA. These data distinguish the NPR1 BTB/POZ domain from other known BTB/POZ domains and establish its molecular role in the context of the Arabidopsis PR-1 gene enhanceosome. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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