التفاصيل البيبلوغرافية
| العنوان: |
Construction of a direct starch-fermenting industrial strain of Saccharomyces cerevisiae producing glucoamylase, α-amylase and debranching enzyme. |
| المؤلفون: |
Ji-Hye Kim, Ha-Ram Kim, Mi-Hyeon Lim, Hyun-Mi Ko, Jong-Eon Chin, Hwanghee Lee, Il-Chul Kim, Suk Bai |
| المصدر: |
Biotechnology Letters; May2010, Vol. 32 Issue 5, p713-719, 7p, 1 Diagram, 2 Charts, 3 Graphs |
| مصطلحات موضوعية: |
SACCHAROMYCES cerevisiae, AMYLODEXTRINS, LEAVENING agents, CELL nuclei, BIOCHEMICAL engineering |
| مستخلص: |
To develop a strain of Saccharomyces cerevisiae that produces ethanol directly from starch, two integrative vectors were constructed to allow the simultaneous multiple integration of the Aspergillus awamori glucoamylase gene ( GA1) and the Debaryomyces occidentalis α-amylase gene ( AMY) and glucoamylase with debranching activity gene ( GAM1) into the chromosomes of an industrial strain of S. cerevisiae. The GA1 and AMY genes were constitutively expressed under the ADC1 promoter in S. cerevisiae using the double δ-integration system. The GAM1 gene was constitutively expressed under the corresponding promoter using the double 18S rDNA-integration system. The recombinant industrial strain secreting biologically active α-amylase, glucoamylase and debranching enzyme was able to ferment starch to ethanol in a single step. The new strain produced 8% (v/v) ethanol (62.8 g l−1) from 20% (w/v) soluble starch after 2 days, fermentation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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