التفاصيل البيبلوغرافية
| العنوان: |
Targeted gene therapy of nasopharyngeal cancer in vitro and in vivo by enhanced thymidine kinase expression driven by human TERT promoter and CMV enhancer. |
| المؤلفون: |
Cong-Xiang Shen, Zhong Wen, Yu-Hong Qian, Shao-Feng Mu, Xiao-Fang Guan |
| المصدر: |
Journal of Experimental & Clinical Cancer Research (17569966); 2010, Vol. 29, p94-102, 9p |
| مصطلحات موضوعية: |
GENE therapy, THYMIDINE, PYRIMIDINE nucleotides, CANCER research, CANCER treatment, CANCER cells, GENETIC vectors, ANTIVIRAL agents |
| مستخلص: |
Background/Aim: To explore the therapeutic effects of thymidine kinase (TK) expressed by enhanced vector pGL3- basic- hTERTp-TK-EGFP-CMV driven by human telomerase reverse transcriptase promoter (hTERTp) as well as cytomegalovirus immediate early promoter enhancer (CMV). Materials/Methods: Enhanced TK-EGFP expression was confirmed by fluorescent microscopy, real time PCR and telomerase activity. Its effects were examined by survival of tumor cells NPC 5-8F and MCF-7, index of xenograft implanted in nude mice and histology. Results: Compared with non-enhanced vector pGL3-basic-TK-hTERTp-EGFP, TK expressed by the enhanced vector significantly decreased NPC 5-8F and MCF-7 cell survival rates after ganciclovir (GCV) treatment (p < 0.001) and tumor progress in nude mice with NPC xenograft and treated with GCV, without obvious toxicity to mouse liver and kidney. Conclusion: The enhanced TK expression vector driven by hTERTp with CMV enhancer has brighter clinical potentials in nasopharyngeal carcinoma therapy than the non-enhanced vector. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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