التفاصيل البيبلوغرافية
| العنوان: |
Increased secretion of TGF-β1 by peripheral blood mononuclear cells from patients with Type 1 diabetes mellitus with diabetic nephropathy. |
| المؤلفون: |
Korpinen, E., Groop, P. -H., Fagerudd, J. A., Teppo, A. -M., Åkerblom, H. K., Vaarala, O. |
| المصدر: |
Diabetic Medicine; Feb2001, Vol. 18 Issue 2, p121-125, 5p, 1 Chart, 3 Graphs |
| مصطلحات موضوعية: |
TRANSFORMING growth factors-beta, MACROPHAGES, DIABETES |
| مستخلص: |
Summary Aims To study transforming growth factor (TGF)-β1 secretion by peripheral blood mononuclear cells (PBMC) from Type 1 diabetic patients with and without nephropathy. Methods Thirty normoalbuminuric Type 1 diabetic patients (urinary albumin excretion rate (AER) < 20 μg/min), 12 microalbuminuric (AER 20–200 μg/min), 10 nephropathic (AER > 200 μg/min), and 13 non-diabetic individuals were recruited. TGF-β1 secretion by PBMC was measured by enzyme immunoassay (EIA) after 48 h culture with and without phytohaemagglutinin (PHA) (5 μg/ml). Results After 48 h culture, the highest TGF-β1 levels secreted by unstimulated PBMC were found in patients with nephropathy (median 6.2 (range 0.9–20.0) ng/ml) when compared to patients with normal albumin excretion (4.1 (0.2–11.3) ng/ml), microalbuminuria (1.8 (0.2–6.4) ng/ml) and healthy controls (1.0 (0.2–7.0) ng/ml); P = 0.02 for the three diabetic groups and P = 0.006 for all groups. At 48 h, the PHA-stimulated TGF-β1 levels were 12.4 (2.9–30.0) ng/ml in nephropathic, 7.3 (0.5–21.2) ng/ml in normoalbuminuric, and 5.5 (0.5–27.6) ng/ml in microalbuminuric patients (P = 0.05). A correlation was observed between TGF-β1 and diastolic blood pressure in the subgroup of patients with incipient and overt nephropathy (r = 0.45, P = 0.04). Conclusions Type 1 diabetic patients with overt nephropathy show increased TGF-β1 secretion by PBMC. Diastolic blood pressure levels correlated with TGF-β1 secretion in dyabetic patients with nephropathy. Increased TGF-β1 secretion by PBMC may be associated with renal and vascular disease in Type 1 diabetes mellitus. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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