التفاصيل البيبلوغرافية
| العنوان: |
α-Conotoxin ImI Inhibits the α-Bungarotoxin-Resistant Nicotinic Response in Bovine Adrenal Chromaffin Cells. |
| المؤلفون: |
Broxton, N. M., Down, J. G., Gehrmann, J., Alewood, P. F., Satchell, D. G., Livett, B. G. |
| المصدر: |
Journal of Neurochemistry; Apr99, Vol. 72 Issue 4, p1656-1662, 7p, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
NICOTINIC receptors, CATECHOLAMINES, CHROMAFFIN cells, SECRETION |
| مستخلص: |
Abstract: The activity of α-conotoxin (α-CTX), ImI, from the vermivorous marine snail Conus imperialis, has been studied on mammalian nicotinic receptors on bovine chromaffin cells and at the rat neuromuscular junction. Synthetic α-CTX ImI was a potent inhibitor of the neuronal nicotinic response in bovine adrenal chromaffin cells (IC50 = 2.5 μM, log IC50 = 0.4 ± 0.07), showing competitive inhibition of nicotine-evoked catecholamine secretion. α-CTX ImI also inhibited nicotine-evoked 45Ca2+ uptake but not 45Ca2+ uptake stimulated by 56 mM K+. In contrast, α-CTX ImI had no effect at the neuromuscular junction over the concentration range 1-20 μM. Bovine chromaffin cells are known to contain the α3β4, α7, and (possibly) α3β4α5 subtypes. However, the secretory response of bovine chromaffin cells is not inhibited by α-bungarotoxin, indicating that α7 nicotinic receptors are not involved. We propose that α-CTX ImI interacts selectively with the functional (α3β4 or α3β4α5) nicotinic acetylcholine receptor to inhibit the neuronal-type nicotinic response in bovine chromaffin cells. [ABSTRACT FROM AUTHOR] |
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