التفاصيل البيبلوغرافية
| العنوان: |
THE DESIGN AND PARTIAL ANALYSIS OF RNAseIII-ANTI-PVS ANTISENSE COMPLEX SYSTEM TO INDUCE PLANT RESISTANCE. |
| المؤلفون: |
Matousek, Jaroslav, Schubert, Jorg, Kuchar, Milan, Dedic, Petr, Ptacek, Jiri, Vrba, Lukas, Lichtenstein, Conrad P. |
| المصدر: |
Archives of Phytopathology & Plant Protection; Feb2001, Vol. 33 Issue 5, p381, 14p |
| مصطلحات موضوعية: |
ANTISENSE RNA, PROTEIN binding, PLANT genetics, PLANT resistance to viruses, DISEASE resistance of plants, POTATO disease & pest resistance |
| مستخلص: |
A system combining antisense RNA and RNAseIII-binding activity was designed. Two antisense RNA vehicles, 7SL RNA and tRNA genes containing the coat protein leader domain (2245-2316) and "zinc finger" domain (3325 3411) of PVS, respectively were prepared. Both, antisense RNA vehicles were shown to be efficiently transcribed by polymerase III using in vitro assay. It was shown by specific RT PCR, that these genes are also transcribed in potato tissues providing that no polymerase II genes are closely linked to them, suggesting some interaction between polll and pollll transcription complexes. Transgenic potato (S. tuberosum cv. Kamyk) lines expressing mutated bacterial RNAseIII, were prepared. From 25 original lines Four had unchanged morphology and ability to grow vigorously in greenhouse conditions and two of them showed lower infection rate than corresponding untransformed control plants after primary infection with PVS. These differences were not observed on plants after the secondary PVS infection. Selected RNAseIII potato lines are currently being transformed with the antisense genes to verify the possibility of induction of plant resistance to potato virus S. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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