التفاصيل البيبلوغرافية
| العنوان: |
High-Resolution Magnetic Resonance Imaging Quantitatively Detects Individual Pancreatic Islets. |
| المؤلفون: |
Lamprianou, Smaragda, Immonen, Riikka, Nabuurs, Christine, Gjinovci, Asllan, Vinet, Laurent, Montet, Xavier C. R., Gruetter, Rolf, Meda, Paolo |
| المصدر: |
Diabetes; Nov2011, Vol. 60 Issue 11, p2853-2860, 8p, 3 Color Photographs, 2 Graphs |
| مصطلحات موضوعية: |
LIVER cells, MAGNETIC resonance imaging, PHAGOCYTES, MITOCHONDRIA, OXIDASES, MICE |
| مستخلص: |
OBJECTIVE--We studied whether manganese-enhanced high- field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes. RESEARCH DESIGN AND METHODS--Whole pancreata from untreated (n = 3), MnCl2 and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl2 and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections. RESULTS--In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of β-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice. CONCLUSIONS--Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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